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  • Huang, Ying  (41)
  • Yang, Jing  (41)
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  • 1
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 71, No. 3 ( 2019-06-06)
    Abstract: Four Gram-stain-positive, catalase-negative, non-spore-forming, rod-shaped bacterial strains (zg-325 T , zg329, dk561 T and dk752) were isolated from the respiratory tract of marmot ( Marmota himalayana ) and the faeces of Tibetan gazelle ( Procapra picticaudata ) from the Qinghai-Tibet Plateau of PR China. The results of 16S rRNA gene sequence-based phylogenetic analyses indicated that strains zg-325 T and dk561 T represent members of the genus Actinomyces , most similar to Actinomyces denticolens DSM 20671 T and Actinomyces ruminicola B71 T , respectively. The DNA G+C contents of strains zg-325 T and dk561 T were 71.6 and 69.3 mol%, respectively. The digital DNA–DNA hybridization values of strains zg-325 T and dk561 T with their most closely related species were below the 70 % threshold for species demarcation. The four strains grew best at 35 °C in air containing 5 % CO 2 on brain heart infusion (BHI) agar with 5 % sheep blood. All four strains had C 18:1 ω9 c and C 16:0 as the major cellular fatty acids. MK-8 and MK-9 were the major menaquinones in zg-325 T while MK-10 was predominant in dk561 T . The major polar lipids included diphosphatidylglycerol and phosphatidylinositol. On the basis of several lines of evidence from phenotypic and phylogenetic analyses, zg-325 T and dk561 T represent novel species of the genus Actinomyces , for which the name Actinomyces marmotae sp. nov. and Actinomyces procaprae sp. nov. are proposed. The type strains are zg-325 T (=GDMCC 1.1724 T =JCM 34091 T ) and dk561 T (=CGMCC 4.7566 T =JCM 33484 T ). We also propose, on the basis of the phylogenetic results herein, the reclassification of Actinomyces liubingyangii and Actinomyces tangfeifanii as Boudabousia liubingyangii comb. nov. and Boudabousia tangfeifanii comb. nov., respectively.
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2019
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  • 2
    Online Resource
    Online Resource
    Microbiology Society ; 2020
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 70, No. 5 ( 2020-05-01), p. 3186-3193
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 5 ( 2020-05-01), p. 3186-3193
    Abstract: Two Gram-stain-negative, strictly aerobic, bright-yellow-pigmented and rod-shaped bacteria (strains 100069 and 100111 T ) with a single polar flagellum were isolated from the rectal contents of plateau pika ( Ochotona curzoniae ). Based on the results of nearly full-length 16S rRNA gene sequence and phylogenetic analyses, strains 100069 and 100111 T belong to the genus Luteimonas , and are closest to Luteimonas rhizosphaerae 4-12 T (98.02 % similarity), Luteimonas aestuarii B9 T (97.8 %) and Luteimonas terrae THG-MD21 T (97.74 %). The DNA G+C contents of these two isolates were 68.30 mol% and 68.29 mol%, respectively. The highest average nucleotide identity (ANI) value between strain 100111 T and its closely related species was 83.34 %, well below the threshold of 95–96 %. The major cellular fatty acids were iso-C 11 : 0 , iso-C 15 : 0 and iso-C 17 : 1   ω9. Polar lipid content was dominated by diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. Ubiquinone-8 (Q-8) was the predominant respiratory quinone. These two isolates grew optimally at 35–37 °C, pH 7.0–8.0 and with 1.0 % (w/v) NaCl. The results of ANI analysis and other characteristics obtained from our polyphasic study showed that strains 100069 and 100111 T represent a novel species in genus Luteimonas , for which the name Luteimonas chenhongjianii sp. nov. (type strain 100111 T =DSM 104077 T =CGMCC 1.16429 T ) is proposed.
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2020
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Microbiology Society ; 2020
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 70, No. 2 ( 2020-02-01), p. 1007-1014
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 2 ( 2020-02-01), p. 1007-1014
    Abstract: Two strains (S-1072 T and 1626) of Gram-stain-negative, oxidase- and catalase-positive, aerobic, rod-shaped, motile bacteria with a single polar flagellum, were isolated from the leaves of Dandelion ( Taraxacum officinale ) on the Qinghai–Tibet Plateau of China. The cells grew optimally at 28 °C, pH 7.0 and with 0.5 % (w/v) NaCl on brain–heart infusion agar. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains S-1072 T and 1626 belong to the genus Luteimonas , sharing the highest similarity with Luteimonas arsenica CCTCC AB 2014326 T (97.0 %), Luteimonas terricola CGMCC 1.8985 T (96.9 %) and Luteimonas aestuarii KCTC 22048 T (96.6 %). The phylogenomic tree indicated that strains S-1072 T and 1626 were most closely related to Luteimonas abyssi CGMCC 1.12611 T . The biochemical characteristics revealed that strains S-1072 T and 1626 could neither produce trypsin nor produce acid from d -glucose, N -acetylglucosamine and maltose, distinguishing them from four closest relatives. The DNA G+C contents of strains S-1072 T and 1626 were 69.2 and 69.3 mol% respectively. The digital DNA–DNA hybridization values of our isolates with their four closely related species were below the 70 % threshold. The predominant menaquinone was Q-8 (98.7 %) and the major polar lipids included diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids ( 〉 10 %) were iso-C 15 : 0 , iso-C 16 : 0 and summed feature 9 (10-methyl C 16 : 0 and/or iso-C 17 : 1 ω 9 c ). Based on the data obtained, strains S-1072 T and 1626 should be classified as a novel species of the genus Luteimonas , for which the name Luteimonas yindakuii sp. nov. is proposed. The type strain is S-1072 T (=CGMCC 1.13927 T =JCM 33487 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2020
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
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  • 4
    Online Resource
    Online Resource
    Microbiology Society ; 2020
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 70, No. 4 ( 2020-04-01), p. 2247-2253
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 4 ( 2020-04-01), p. 2247-2253
    Abstract: Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-A T and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai–Tibet Plateau. Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C. The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus , respectively. In the 16S rRNA gene and polygenetic trees, strains MF30-A T and MF845 were clearly distinct from other species. The two strains could not produce acid from arbutin, d -fructose, D-sucrose, glycogen, salicin or starch. Production of β-glucosidase by these strains was negative. The major fatty acids of these strains were anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 . Strain MF30-A T contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones. The major polar lipids in strain MF30-A T were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid. The G+C contents of the DNA of strains MF30-A T and MF845 were 69.8 mol% and 69.7 mol%, respectively. The average nucleotide identity and digital DNA–DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively. All genotypic and phenotypic data indicated that strains MF30-A T and MF845 should be classified as novel members of the genus Agromyces , for which the name Agromyces badenianii sp. nov. is proposed. The type strain is MF30-A T (=CGMCC 1.16469 T =DSM 106183 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2020
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Microbiology Society ; 2020
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 70, No. 6 ( 2020-06-01), p. 3665-3672
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 6 ( 2020-06-01), p. 3665-3672
    Abstract: Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136 T and dk3543, were isolated from the faeces of Tibetan gazelle ( Procapra picticaudata ) in the Qinghai–Tibet Plateau of PR China. The cells were aerobic, oxidase-negative and catalase-positive. Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain–heart infusion (BHI) agar with 5 % sheep blood. The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood. Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25 T (97.9 % similarity). The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25 T , Nocardioides gilvus XZ17 T , Nocardioides houyundeii 78 T and Nocardioides daphniae D287 T were their nearest phylogenetic neighbours. The DNA G+C contents of strains dk3136 T and dk3543 were 70.3 mol% and 70.4 mol%, respectively. Their genomes exhibit lower than threshold (95–96 %) average nucleotide identity to known species of the genus Nocardioides . ll -2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H 4 ) was the predominant respiratory quinone. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The two strains had C 18 : 1 ω 9 c , iso -C 16 : 0 and C 17 : 1 ω 8 c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars. On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136 T and dk3543 represent a novel species in genus Nocardioides , for which the name Nocardioides jishulii sp. nov. is proposed. The type strain is dk3136 T (=CGMCC 4.7570 T =JCM 33496 T =KCTC 49314 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2020
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 6
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 71, No. 1 ( 2021-01-01)
    Abstract: Six aerobic, non-motile, non-haemolytic, Gram-stain-negative, oxidase-negative strains (185 T , 187, 323-1 T , 194, dk386 T and dk771) were recovered from different faecal samples of Equus kiang on the Qinghai–Tibet Plateau. In the 16S rRNA gene sequences, one strain pair, 185 T /187, shared highest similarity to Acinetobacter equi 114 T (97.9 %), and the other two (323-1 T /194 and dk771 T /dk386) to Acinetobacter harbinensis CGMCC 1.12528 T (98.6 and 97.0 %, respectively). Phylogenomic tree analysis showed that these six strains formed three separate clades in the genus Acinetobacter . Digital DNA–DNA hybridization values of each pair of the isolates with all members of the genus Acinetobacter were far below 70 %. The main cellular fatty acids of all six strains were C 18 : 1  ω 9 c , C 16 : 0 and summed feature 3 (C 16 : 1  ω 7 c /C 16 : 1  ω 6 c ). Q-9 was the predominant respiratory quinone for strains 185 T , 323-1 T and dk386 T . The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the genotypic, phenotypic and biochemical analyses, these six strains represent three novel species of the genus Acinetobacter , for which the names Acinetobacter lanii sp. nov., Acinetobacter shaoyimingii sp. nov. and Acinetobacter wanghuae sp. nov. are proposed. The type strains are 185 T (=CGMCC 1.13636 T =JCM 33607 T ), 323-1 T (=CGMCC 1.13940 T =JCM 33608 T ) and dk386 T (=CGMCC 1.16589 T =JCM 33592 T ), respectively.
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2021
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
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  • 7
    In: Journal of Microbiology, Springer Science and Business Media LLC, Vol. 60, No. 2 ( 2022-02), p. 147-155
    Type of Medium: Online Resource
    ISSN: 1225-8873 , 1976-3794
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2441367-7
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  • 8
    Online Resource
    Online Resource
    Microbiology Society ; 2019
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 69, No. 11 ( 2019-11-01), p. 3485-3491
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 69, No. 11 ( 2019-11-01), p. 3485-3491
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2019
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Microbiology Society ; 2020
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 70, No. 2 ( 2020-02-01), p. 744-750
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 2 ( 2020-02-01), p. 744-750
    Abstract: Two strains, designated 2251 T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau. The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251 T and 3058 represent a new species within the genus Paracoccus and are most similar to ‘ Paracoccus gahaiensis ’ CUG00006 T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45 T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31 T (97.6 and 97.8 %). Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis. Optimal growth was achieved at pH 7.0–7.5 and 28 °C with marine medium. Cells contained C 18 : 1 ω 7 c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone. The polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phospholipid, glycolipid and an unidentified lipid. The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose. The G+C content of strain 2251 T was 66.5 mol%. Both strains (2251 T and 3058) had DNA–DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database. Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251 T and 3058 should be classified as a novel species of the genus Paracoccus , for which the name Paracoccus liaowanqingii sp. nov. is suggested. The type strain is 2251 T (=CGMCC 1.16490 T =DSM 106269 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2020
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 10
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 8 ( 2020-08-01), p. 4683-4690
    Abstract: Four novel strains (592 T , S592, MF47 T and SMF47) were isolated from Tibetan antelopes ( Pantholops hodgsonii ) and plateau pikas ( Ochotona curzoniae ), respectively. The cells were aerobic, non-motile, Gram-stain- and catalase-positive, rod-shaped bacteria. The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552 T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592 T and MF47 T represent two novel species. The four isolates produced acid from l -rhamnose, d -xylose and cellobiose, but were unable to reduce nitrate. The DNA G+C contents of strains 592 T and MF47 T were 70.3 and 69.8 mol%, respectively. The digital DNA–DNA hybridization value between strains 592 T and MF47 T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species. The four strains’ genomes displayed less than 24.6 % DNA–DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897 T and Aeromicrobium ginsengisoli JCM 14732 T . The major fatty acids of the four strains were C 18 : 1   ω9 c and C 18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant respiratory quinones were MK-9(H 4 ) and MK-8(H 4 ). The cell-wall peptidoglycan contained ll -diaminopimelic acid. Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp. nov. and Aeromicrobium yanjiei sp. nov. The type strains are 592 T (=CGMCC1.16526 T =DSM 106289 T ) and MF47 T (=CGMCC 1.17444 T =JCM 33790 T ), respectively.
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2020
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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