GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    A Novel PCR-Based Functional Marker of Rice Blast Resistance Gene Pi25
    MDPI AG ; 2023
    In:  Agriculture Vol. 13, No. 10 ( 2023-09-30), p. 1926-
    Details
    In: Agriculture, MDPI AG, Vol. 13, No. 10 ( 2023-09-30), p. 1926-
    Abstract: Rice blast is arguably the most devastating fungal disease of rice. Utilization of resistance genes to breed resistant cultivars is one of the most economical and environmentally friendly approaches to combat the disease. Pi25, a major resistance gene conferring broad-spectrum resistance to both leaf and neck blast, is an ideal gene resource to improve the resistance of rice varieties to blast. Recently, several allele-specific markers were developed. However, they were deficiently efficient due to either an additional process of restriction enzyme digestion for cleaved amplified polymorphic sequence (CAPS) markers or the risk of false-positive error in identifying susceptible Tetep allele (Pi25TTP) for PCR-based markers. In this study, based on a conserved single nucleotide polymorphism (SNP) between resistant and susceptible alleles, a tetra-primer amplification refractory mutation system (ARMS)-PCR marker was developed. The new marker, namely Pi25-2687R3, could effectively distinguish the resistant Gumei 2 (GM2) allele (Pi25GM2) and the susceptible allele Pi25TTP. Moreover, a perfect consistency of genotyping was exhibited between Pi25-2687R3 and published CAPS marker CAP3/Hpy 99I. A more accurate genotyping was also displayed compared to the previous PCR-based SNP marker Pi25-2566. Our finding proved that Pi25-2687R3 could achieve the same result as CAP3/Hpy 99I with less workload and cost and could promote the accuracy in the identification of genotypes superior to Pi25-2566. This study provided a quick and reliable functional marker for discriminating Pi25 alleles, which would be a valuable tool for genotypic assay and rice molecular breeding of blast resistance.
    Type of Medium: Online Resource
    ISSN: 2077-0472
    URL: Article
    DOI: 10.3390/agriculture13101926
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2651678-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Dissection of two quantitative trait loci for grain weight linked in repulsion on the long arm of chromosome 1 of rice (Oryza sativa L.)
    Elsevier BV ; 2013
    In:  The Crop Journal Vol. 1, No. 1 ( 2013-10), p. 70-76
    Details
    In: The Crop Journal, Elsevier BV, Vol. 1, No. 1 ( 2013-10), p. 70-76
    Type of Medium: Online Resource
    ISSN: 2214-5141
    URL: Article
    DOI: 10.1016/j.cj.2013.07.008
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2013
    detail.hit.zdb_id: 2745450-2
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Detection of QTL for High-Temperature Tolerance in Rice Using a High-Density Bin Map
    MDPI AG ; 2023
    In:  Agronomy Vol. 13, No. 6 ( 2023-06-12), p. 1582-
    Details
    In: Agronomy, MDPI AG, Vol. 13, No. 6 ( 2023-06-12), p. 1582-
    Abstract: Rice is sensitive to high-temperature stress during almost all stages of growth and development. High-temperature stress has become one of the main factors restricting high yield and superior quality of rice. In this study, recombinant inbred lines (RILs) derived from an indica rice cross between two restorer lines were planted in two years. One sowing date was applied in 2019, and four sowing dates were set in 2020 according to the period of local high temperatures in recent years. Two traits closely related to high-temperature tolerance, heading date (HD), and spikelet fertility (SF) were measured. In each trial, the HD showed a bimodal distribution, whereas SF had a continuous and left-skewed distribution. QTL analysis was performed using a high-density bin map. For HD, a total of six QTL were detected. All of them correspond in position to the cloned genes, among which qHD8 in the DTH8/Ghd8 region showed the largest genetic effect. For SF, a total of eight QTL were detected. Five of them, qSF1, qSF2, qSF3.1, qSF3.2, and qSF8, showed high-temperature tolerance and had an important potential in rice breeding.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    URL: Article
    DOI: 10.3390/agronomy13061582
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2607043-1
    SSG: 23
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    QTL detection for grain yield and micro-nutrition contents in rice (
    CSIRO Publishing ; 2022
    In:  Crop & Pasture Science Vol. 73, No. 11 ( 2022-5-24), p. 1253-1263
    Details
    In: Crop & Pasture Science, CSIRO Publishing, Vol. 73, No. 11 ( 2022-5-24), p. 1253-1263
    Abstract: Context Enhancing grain yield and micro-nutrition contents in rice (Oryza sativa L.) through biofortification breeding is an efficient way to address micro-nutrient deficiencies in human. Aims QTL mapping for grain yield and micro-nutritional traits is prerequisite for developing new rice varieties. Methods QTLs for grain yield and micro-nutritional traits were identified using a backcross inbred lines (BIL) derived from an interspecific backcross of Xieqingzao B and Dongxiang wild rice (Oryza rufipogon Griff.). Key results A total of 16 and 29 QTLs were identified for grain Zn, Mn and Cu contents, including three for brown and milled rice, 10 for brown rice only, and three for milled rice only, and for grain related traits, respectively. Among grain micro-nutrient QTLs, three QTLs, qMn4, qMn6.2 and qZn6.2, showed genetic effects on both brown and milled rice. Conclusion Sixteen QTLs for grain related traits and eight QTLs for grain micro-nutrient content had O. rufipogon favourable alleles. A total 31 QTLs were clustered eight chromosomal regions. Importantly, two clusters, qZn4/qGW4 and qZn6.2/qMn6.2/qCu6.3/qGYP6.2, had the favourable wild alleles, suggesting that O. rufipogon alleles had synergistic effects on both yield component and micro-nutrient content. Implications These candidate QTLs could be useful for the improvement of grain yield and micro-nutrients through QTL pyramiding.
    Type of Medium: Online Resource
    ISSN: 1836-0947 , 1836-5795
    URL: Article
    DOI: 10.1071/CP22039
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...