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  • Hindawi Limited  (18)
  • Huang, Chao  (18)
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  • Hindawi Limited  (18)
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  • 1
    Online Resource
    Online Resource
    Polyphyllin VII Promotes Apoptosis and Autophagic Cell Death via ROS-Inhibited AKT Activity, and Sensitizes Glioma Cells to Temozolomide
    Hindawi Limited ; 2019
    In:  Oxidative Medicine and Cellular Longevity Vol. 2019 ( 2019-11-14), p. 1-19
    Details
    In: Oxidative Medicine and Cellular Longevity, Hindawi Limited, Vol. 2019 ( 2019-11-14), p. 1-19
    Abstract: The high recurrence frequency of gliomas but deficiency of effective treatment and prevalent chemoresistance have elicited interests in exploring and developing new agents. Paris polyphyllins are monomers extracted from rhizome of Paris polyphylla var. yunnanensis. Here, we first reported that polyphyllin VII (PP7) exhibited cytotoxic effect on glioma cells. PP7 significantly suppressed the viability and induced cell death of U87-MG and U251 cells after 24 h, with the IC50 values 4.24 ± 0.87   μ M and 2.17 ± 0.14   μ M , respectively. Both apoptotic and autophagic processes were involved in the cytotoxic effect of PP7, as PP7 activated the Bcl2/Bax pathway and the inhibition of autophagy partly rescued the toxicity of PP7 in glioma cells. In addition, an inhibition of AKT/mTORC1 activity was found after PP7 administration, and it seemed that the overproduction of reactive oxygen species (ROS) was responsible for this effect. Namely, the removal of ROS by NAC treatment mitigated PP7-induced cell death, autophagy, and its effect on the AKT/mTORC1 signaling. Additionally, a combination assay of PP7 with temozolomide (TMZ), the most used chemotherapy for glioma patients, was performed resulting in synergism, while PP7 reduced TMZ resistance through inhibition of MGMT expression. Thus, our study reports PP7 as a potential agent for glioma treatment and reveals its underlying mechanisms of action.
    Type of Medium: Online Resource
    ISSN: 1942-0900 , 1942-0994
    URL: Article
    DOI: 10.1155/2019/1805635
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2455981-7
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  • 2
    Online Resource
    Online Resource
    miR-155 Knockdown Protects against Cerebral Ischemia and Reperfusion Injury by Targeting MafB
    Hindawi Limited ; 2020
    In:  BioMed Research International Vol. 2020 ( 2020-01-22), p. 1-11
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2020 ( 2020-01-22), p. 1-11
    Abstract: Background . Studies have elucidated that the variable expression levels of miRNAs influence the inflammatory process in ischemic stroke. Nevertheless, the impact and potential mechanism of miR-155 in cerebral ischemia-reperfusion injury (CIRI) keep to be incompletely known. Methods . The levels of miR-155 and MafB were determined via qRT-PCR, western blot, or immunohistochemistry assays in plasma of patients with CIRI, oxygen glucose deprivation/reoxygenation (OGD/R) induced SH-SY5Y cells, and mouse models with middle cerebral artery occlusion (MCAO). The association between miR-155 and MafB was validated via dual-luciferase reporter and western blot assays. Cell viability, apoptosis, invasion, and migration were evaluated through MTT, flow cytometry, Transwell and wound healing assays. Infarction volume was measured in MCAO mouse brain tissues by TTC assay. The expression of inflammatory mediators was measured by ELISA in cells and brain tissues. Results . miR-155 level was upregulated whereas MafB was downregulated in the plasma of patients with CIRI, OGD/R-induced SH-SY5Y cells, also as mouse models with MCAO injury. Mechanistically, miR-155 directly targeted 3’UTR of MafB and restrained MafB expression in OGD/R injury SH-SY5Y cells. Downregulation of miR-155 attenuated OGD/R-induced injury through increasing proliferation, inhibiting apoptosis, enhancing invasion and migration abilities, and constraining the expression of inflammatory mediators (IL-1 β , IL-6, and TNF- α ) and inflammatory enzymes (iNOS and COX-2) in SH-SY5Y cells following OGD/R, while MafB inhibition reversed the protective effects. In vivo , downregulating miR-155 reduced the infarction volume in the MACO mouse brain. Furthermore, miR-155 knockdown inhibited the IL-1 β , IL-6, TNF- α , iNOS, and COX-2 in the MACO mouse brain tissues. Conclusion . Our results suggest that miR-155 knockdown alleviated ischemia-reperfusion injury by targeting MafB to improve the neurological function and inhibit inflammation response, highlighting a novel therapeutic strategist for CIRI.
    Type of Medium: Online Resource
    ISSN: 2314-6133 , 2314-6141
    URL: Article
    DOI: 10.1155/2020/6458204
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2698540-8
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  • 3
    Online Resource
    Online Resource
    Quantitative Scintigraphy Evaluated the Relationship between 131I Therapy and Salivary Glands Function in DTC Patients: A Retrospective Analysis
    Hindawi Limited ; 2022
    In:  Journal of Healthcare Engineering Vol. 2022 ( 2022-4-14), p. 1-13
    Details
    In: Journal of Healthcare Engineering, Hindawi Limited, Vol. 2022 ( 2022-4-14), p. 1-13
    Abstract: Purpose. Quantitative scintigraphy to evaluate salivary gland function changes in patients with differentiated thyroid cancer (DTC) after iodine-131 (131I) treatment. Methods. A total of 458 patients with DTC grouped by sex and age were included. Salivary gland scintigraphy was performed to evaluate salivary gland function before and after 131I treatment. The uptake fraction (UF), uptake index (UI), and excretion fraction (EF) of two pairs of parotid glands and submandibular glands were measured and compared. The Chi-square test was conducted according to function impairment count. Results. Salivary gland function in different age groups and sexes were quite different, especially for women 〈 55 years old, who had decreased UF, UI, and EF of all four glands without basal injury. The secretion or uptake function of some salivary glands with basic function impairment before 131I treatment was increased after iodine treatment. Only a small percentage of males showed reduced functional parameters after several treatments. The most significant difference in the count of impairment for the four salivary glands were the first and third examinations, which was more evident in women. The submandibular gland had the most significant reduction in uptake. Conclusion. Changes in salivary gland function are more common in young females being treated for DTC. Impairment of salivary gland function is correlated with the number of treatments and the cumulative dose of 131I. Some salivary gland functions impaired before 131I treatment were enhanced in the early treatment.
    Type of Medium: Online Resource
    ISSN: 2040-2309 , 2040-2295
    URL: Article
    DOI: 10.1155/2022/7640405
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2545054-2
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  • 4
    Online Resource
    Online Resource
    Knockdown of Fibromodulin Inhibits Proliferation and Migration of RPE Cell via the VEGFR2-AKT Pathway
    Hindawi Limited ; 2018
    In:  Journal of Ophthalmology Vol. 2018 ( 2018-09-12), p. 1-8
    Details
    In: Journal of Ophthalmology, Hindawi Limited, Vol. 2018 ( 2018-09-12), p. 1-8
    Abstract: Purpose . Recent research has provided novel insight into the function of fibromodulin (FMOD) in wound healing and angiogenesis. The role of FMOD in initiation of proliferative vitreoretinopathy (PVR) has not been studied. This study investigated the effect of FMOD on human retinal pigment epithelial (RPE) cell, which plays an essential role in the progression of PVR, and the possible mechanisms. Methods . Small interfering (si) RNA-based gene transfer technology was used to decrease FMOD expression and to study its effects on RPEs in vitro . Cell Counting Kit-8 assays, transwells, and flow cytometry analysis were used to measure cell proliferation, migration, cell cycle, and apoptosis. Western blot was used to measure expression of vascular endothelial growth factor (VEGF), VEGF receptor 2 (VEGFR2), extracellular signal-related kinase 1/2 (ERK1/2), and phosphoinositide 3 kinase (PI3K/AKT). Results . After transfection of RPEs with a FMOD-specific siRNA, cell proliferation and migration were inhibited to the percentage of 65% ± 5% and 39% ± 10%, respectively, compared to the control group. Depletion of FMOD induced cell cycle arrest and apoptosis in RPE cells. Downregulation of VEGF, VEGFR2, and phosphorylated AKT (p-AKT) were detected in transfected RPEs. Conclusion . Depletion of FMOD selectively downregulated the expression of VEGF and VEGFR2 and inhibited the signaling pathway of AKT phosphorylation, which consequently inhibited the proliferation and migration of RPE Cell.
    Type of Medium: Online Resource
    ISSN: 2090-004X , 2090-0058
    URL: Article
    DOI: 10.1155/2018/5708537
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2018
    detail.hit.zdb_id: 2546525-9
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  • 5
    Online Resource
    Online Resource
    Gender-Specific Differences on the Association of Hypertension with Subclinical Thyroid Dysfunction
    Hindawi Limited ; 2019
    In:  International Journal of Endocrinology Vol. 2019 ( 2019-12-30), p. 1-9
    Details
    In: International Journal of Endocrinology, Hindawi Limited, Vol. 2019 ( 2019-12-30), p. 1-9
    Abstract: Objective . Both hypertension and subclinical thyroid dysfunction (STD) have high prevalence and clinical importance, but their relationship is still a matter of debate. We aimed to explore gender-specific difference on the association between hypertension and STD in Chinese. Methods . We recruited 13,380 ostensible healthy participants (8,237 men and 5,143 women). The associations between hypertension and STD were analyzed on a gender-based setting after dividing STD into subclinical hypothyroidism, subclinical hyperthyroidism and further subgrouped euthyroidism. Crude and adjusted odds ratios of STD for hypertension were analyzed by binary logistic regression. Results . An increasing trend of hypertension prevalence was found along with aging in both genders. Yet, higher male hypertension prevalence was found until 65 years, and then it intersected with female hypertension prevalence. Women had significantly higher propensity for STD than men. Yet, in elderly participants, this gender-specific difference became less obvious. We displayed detrimental effects for subclinical hypothyroidism in both genders after multiple-covariate adjustments, yet no such effects were shown for subclinical hyperthyroidism. Moreover, females with subclinical hypothyroidism were more likely to be associated with hypertension than males, and the corresponding odds ratios were 1.619 ( P 〈 0.01 ) and 1.557 ( P 〈 0.05 ), respectively. For systolic blood pressure, the adjusted odds ratios were 1.371 ( P 〈 0.05 ) and 1.154 ( P 〉 0.05 ), and for diastolic blood pressure, 1.604 ( P 〈 0.01 ) and 1.564 ( P 〈 0.01 ), respectively. Conclusion . We demonstrate that hypertension is associated with subclinical hypothyroidism, but not with subclinical hyperthyroidism. Moreover, females with subclinical hypothyroidism are more likely to be associated with hypertension than males.
    Type of Medium: Online Resource
    ISSN: 1687-8337 , 1687-8345
    URL: Article
    DOI: 10.1155/2019/6053068
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2502951-4
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  • 6
    Online Resource
    Online Resource
    Pincer Nail Deformity: Clinical Characteristics, Causes, and Managements
    Hindawi Limited ; 2020
    In:  BioMed Research International Vol. 2020 ( 2020-04-15), p. 1-15
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2020 ( 2020-04-15), p. 1-15
    Abstract: A pincer nail is a common nail deformity of toenails and is characterized by nail thickening and nail plate deformation. It often causes severe pain for patients. We perform a thorough literature review and an additional review of pertinent clinical cases, aiming to provide a comprehensive review of the etiology, pathogenesis, clinical classification, differential diagnosis, and treatment of pincer nail deformity (PND). Understanding the clinical characteristics and treatment progress of a pincer nail will provide clinicians with comprehensive and evidence-based information about PND, thus allowing the selection of an appropriate treatment according to the patient’s request and the clinical manifestations of PND, which should maximize patient satisfaction.
    Type of Medium: Online Resource
    ISSN: 2314-6133 , 2314-6141
    URL: Article
    DOI: 10.1155/2020/2939850
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2698540-8
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  • 7
    Online Resource
    Online Resource
    Analysis of the Impacts of Bearing on Vibration Characteristics of Rotor
    Hindawi Limited ; 2017
    In:  Shock and Vibration Vol. 2017 ( 2017), p. 1-7
    Details
    In: Shock and Vibration, Hindawi Limited, Vol. 2017 ( 2017), p. 1-7
    Abstract: Aiming at a Top Gas Recovery Turbine Unit (TRT) with double support rotor and the extending disk end, theoretical and experimental analysis about influence of cylindrical bearing and four-lobe bearing on vibration of TRT rotor system are conducted in this paper. The results indicate that vibration of the rotor supported by cylindrical bearing is more stable than that supported by four-lobe bearing at the driving end (DE) and the nondriving end (NDE). The amplitude of rotor is supported by both of these types of bearing increases as the speed increases at the NDE, while the amplitude of the DE remains unchanged. Comparing with the result of theoretical analysis, the practical test results are more consistent with the theoretical response analysis conducted by applying unbalanced mass at the extending disk end. This paper presents an analysis method of the critical characteristics of a double support rotor system with the extending disk end and provides reference value for dealing with vibration fault of double support rotor system with the extending disk end.
    Type of Medium: Online Resource
    ISSN: 1070-9622 , 1875-9203
    URL: Article
    DOI: 10.1155/2017/8098591
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2017
    detail.hit.zdb_id: 2070162-7
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  • 8
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    Online Resource
    Prediction of Visual Acuity after anti-VEGF Therapy in Diabetic Macular Edema by Machine Learning
    Hindawi Limited ; 2022
    In:  Journal of Diabetes Research Vol. 2022 ( 2022-4-19), p. 1-10
    Details
    In: Journal of Diabetes Research, Hindawi Limited, Vol. 2022 ( 2022-4-19), p. 1-10
    Abstract: Purpose. To predict visual acuity (VA) 1 month after anti-vascular endothelial growth factor (VEGF) therapy in patients with diabetic macular edema (DME) by using machine learning. Methods. This retrospective study included 281 eyes with DME receiving intravitreal anti-VEGF treatment from January 1, 2019, to April 1, 2021. Eighteen features from electronic medical records and measurements data from OCT images were extracted. The data obtained from January 1, 2019, to November 1, 2020, were used as the training set; the data obtained from November 1, 2020, to April 1, 2021, were used as the validation set. Six different machine learning algorithms were used to predict VA in patients after anti-VEGF therapy. After the initial detailed investigation, we designed an optimization model for convenient application. The VA predicted by machine learning was compared with the ground truth. Results. The ensemble algorithm (linear regression + random forest regressor) performed best in VA and VA variance predictions. In the validation set, the mean absolute errors (MAEs) of VA predictions were 0.137-0.153 logMAR (within 7-8 letters), and the mean square errors (MSEs) were 0.033-0.045 logMAR (within 2-3 letters) for the 1-month VA predictions, respectively. For the prediction of VA variance at 1 month, the MAEs were 0.164-0.169 logMAR (within 9 letters), and the MSEs were 0.056-0.059 logMAR (within 3 letters), respectively. Conclusions. Our machine learning models could accurately predict VA and VA variance in DME patients receiving anti-VEGF therapy 1 month after, which would be much valuable to guide precise individualized interventions and manage expectations in clinical practice.
    Type of Medium: Online Resource
    ISSN: 2314-6753 , 2314-6745
    URL: Article
    DOI: 10.1155/2022/5779210
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2711897-6
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  • 9
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    Online Resource
    Gastric Cancer Mesenchymal Stem Cells Inhibit NK Cell Function through mTOR Signalling to Promote Tumour Growth
    Hindawi Limited ; 2021
    In:  Stem Cells International Vol. 2021 ( 2021-6-29), p. 1-17
    Details
    In: Stem Cells International, Hindawi Limited, Vol. 2021 ( 2021-6-29), p. 1-17
    Abstract: The dysfunction of natural killer (NK) cells has been increasingly reported in malignancies, especially in solid tumours. Mesenchymal stem cells (MSCs) exhibit pleiotropic functions that include mediating immune cell exhaustion which is implicated in cancer progression. However, the association of MSCs derived from gastric cancer (gastric cancer mesenchymal stem cells: GCMSCs) with the dysfunction of NK cells remains poorly understood. In this study, we demonstrated that GCMSCs effectively contributed to the exhaustion of NK cells through the release of soluble factors. Furthermore, passivation of the antitumour effect in NK cells was closely associated with their dysfunctional state. The GCMSC-conditioned medium prevented the frequency and effector function of infiltrating NK cells in tumour-bearing mouse models, thus promoting tumour growth. Mechanistically, mammalian target of rapamycin (mTOR) signalling, a critical regulator of cellular metabolism that mediates the function of immune cells, was inhibited in NK cells treated with GCMSCs. However, the checkpoint receptor PD-1 was still present at minimal levels with or without GCMSCs. The study results revealed that GCMSCs contributed to dysfunctional NK cells involved at least partially in the inhibition of mTOR signalling, suggesting potential directions for NK cell-based cancer immunotherapy.
    Type of Medium: Online Resource
    ISSN: 1687-9678 , 1687-966X
    URL: Article
    DOI: 10.1155/2021/9989790
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2573856-2
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  • 10
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    Online Resource
    Heat Transfer Enhancement of Energy Pile with Nanofluids as Heat Carrier
    Hindawi Limited ; 2023
    In:  Advances in Civil Engineering Vol. 2023 ( 2023-9-29), p. 1-15
    Details
    In: Advances in Civil Engineering, Hindawi Limited, Vol. 2023 ( 2023-9-29), p. 1-15
    Abstract: As one of the core components of the transport medium in the energy pile system, the circulating working fluid is crucial for the enhancement of its heat transfer efficiency. In this study, based on the heat transfer enhancement theory of nanofluids, deionized water was used as the base solution to prepare the corresponding 0, 0.5, 1, and 1.5 vol% nanofluids by introducing nanoparticles SiO2, Al2O3, CuO, and Cu. The laws of thermal conductivity and specific heat capacity with the volume fraction of different nanofluids are studied by the comparison. The results of thermal conductivity research illustrate that the specific heat capacity of nanofluids decreases linearly with the increase of the volume concentration of nanoparticles, but the thermal conductivity increases linearly. The fluid with the highest thermal conductivity and specific heat capacity is Cu–water nanofluids. In addition, it is proposed to directly apply all kinds of nanofluids with 1 vol% as circulating fluid to self-made miniature model box of energy pile to simulate the working conditions in summer. The influence laws of the nanofluids on the flow rate and heat transfer efficiency in the energy pile were calculated through the temperature difference between the inlet and outlet of the heat exchange tube. The results reveal that the nanofluids used in the experiment exert a optimization influence on the heat transfer of energy pile but the mass flow rate of the nanofluids during the circulation is decreased compared with that of the deionized water circulation liquid. Among them, the temperature difference between inlet and outlet of Cu–water nanofluid is the largest, which has the best effect on improving the heat transfer efficiency of the energy pile but its mass flow loss is also the largest. The research results can provide a reliable reference for the improvement of heat transfer efficiency of energy pile engineering in the future.
    Type of Medium: Online Resource
    ISSN: 1687-8094 , 1687-8086
    URL: Article
    DOI: 10.1155/2023/2035247
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2449760-5
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