In:
Clinical Chemistry, Oxford University Press (OUP), Vol. 44, No. 12 ( 1998-12-01), p. 2441-2445
Abstract:
We established the validity of a quantitative reverse transcription (RT)-PCR assay for the RNA component of human telomerase (hTR), using the TaqMan fluorogenic detection system. Using this assay, we quantified hTR expression in two human pancreatic cancer cell lines, ASPC-1 and MIAPaCa-2. Our results indicated that hTR expression in MIAPaCa-2 was 1.99-fold higher than that in ASPC-1 cells. This TaqMan RT-PCR assay appears to be useful in determining the amount of hTR in clinical specimens.
Type of Medium:
Online Resource
ISSN:
0009-9147
,
1530-8561
DOI:
10.1093/clinchem/44.12.2441
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1998
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