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  • Hill, Martyn  (2)
  • Komparatistik. Außereuropäische Sprachen/Literaturen  (2)
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  • Komparatistik. Außereuropäische Sprachen/Literaturen  (2)
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  • 1
    Online-Ressource
    Online-Ressource
    Acoustical Society of America (ASA) ; 2008
    In:  The Journal of the Acoustical Society of America Vol. 123, No. 5_Supplement ( 2008-05-01), p. 3925-3925
    In: The Journal of the Acoustical Society of America, Acoustical Society of America (ASA), Vol. 123, No. 5_Supplement ( 2008-05-01), p. 3925-3925
    Kurzfassung: A new acoustic/optical/microfluidic system is presented for the manipulation of bead-tagged DNA molecules. Acoustic radiation forces are used to manipulate microspheres into and away from the evanescent field of a laser coupled waveguide that is integrated into the reflector of the acoustic chamber. With suitable fluorophores the presence of the target DNA can be detected with a fluorescence microscope enabling large populations of beads to be examined simultaneously. The integrated waveguide and multimodal acoustic chamber are presented here, with results showing that the microspheres can be successfully detected as they are brought into the evanescent field using a quarter-wave acoustic configuration. It is also shown that by measuring the time of flight of a microsphere between the half- and quarter-wave nodal planes the bead size can be determined, providing a means of multiplexing the detection (detecting a range of different target DNA sequences).
    Materialart: Online-Ressource
    ISSN: 0001-4966 , 1520-8524
    RVK:
    Sprache: Englisch
    Verlag: Acoustical Society of America (ASA)
    Publikationsdatum: 2008
    ZDB Id: 1461063-2
    Standort Signatur Einschränkungen Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 2
    Online-Ressource
    Online-Ressource
    Acoustical Society of America (ASA) ; 2008
    In:  The Journal of the Acoustical Society of America Vol. 123, No. 5_Supplement ( 2008-05-01), p. 3841-3841
    In: The Journal of the Acoustical Society of America, Acoustical Society of America (ASA), Vol. 123, No. 5_Supplement ( 2008-05-01), p. 3841-3841
    Kurzfassung: Prior work [Petersson et al., 2004] has demonstrated the operation of a half-wave acoustic particle manipulator, whose forces act in the plane of a silicon substrate. In a half-wave device particles are directed to the centre of a channel. Devices acting in plane have manufacturing advantages, and lend themselves to many microfluidic applications. We demonstrate, for the first time, such a device with a quarter-wave mode that is able to manipulate particles to the side of a channel in addition to a near half wave mode. The design utilises resonant “islands” to create the necessary pressure release boundary condition. The device is conventionally milled in brass, permitting cheaper and quicker fabrication than in silicon. Finite element modelling is presented to elucidate the operation of both half and quarter-wave devices. In contrast to essentially one-dimensional planar devices, the two-dimensional distribution of the velocity and pressure fields result in particles being constrained to a line within the channel.
    Materialart: Online-Ressource
    ISSN: 0001-4966 , 1520-8524
    RVK:
    Sprache: Englisch
    Verlag: Acoustical Society of America (ASA)
    Publikationsdatum: 2008
    ZDB Id: 1461063-2
    Standort Signatur Einschränkungen Verfügbarkeit
    BibTip Andere fanden auch interessant ...
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