In:
The Journal of the Acoustical Society of America, Acoustical Society of America (ASA), Vol. 123, No. 5_Supplement ( 2008-05-01), p. 3925-3925
Abstract:
A new acoustic/optical/microfluidic system is presented for the manipulation of bead-tagged DNA molecules. Acoustic radiation forces are used to manipulate microspheres into and away from the evanescent field of a laser coupled waveguide that is integrated into the reflector of the acoustic chamber. With suitable fluorophores the presence of the target DNA can be detected with a fluorescence microscope enabling large populations of beads to be examined simultaneously. The integrated waveguide and multimodal acoustic chamber are presented here, with results showing that the microspheres can be successfully detected as they are brought into the evanescent field using a quarter-wave acoustic configuration. It is also shown that by measuring the time of flight of a microsphere between the half- and quarter-wave nodal planes the bead size can be determined, providing a means of multiplexing the detection (detecting a range of different target DNA sequences).
Type of Medium:
Online Resource
ISSN:
0001-4966
,
1520-8524
Language:
English
Publisher:
Acoustical Society of America (ASA)
Publication Date:
2008
detail.hit.zdb_id:
1461063-2
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