In:
The Journal of Immunology, The American Association of Immunologists, Vol. 182, No. 1_Supplement ( 2009-04-01), p. 39.9-39.9
Abstract:
Our previous study showed that sphingosine 1-phosphate (S1P) regulates peritoneal B cell trafficking for the subsequent intestinal IgA production. In this study, we demonstrate that stromal NFkappaB-inducing kinase (NIK) is specifically involved in this pathway. Although peritoneal B cells from NIK-mutated alymphoplasia (aly) mice expressed substantial levels of S1P receptor and showed normal migration toward S1P, aly peritoneal B cells showed decreased sensitivity to FTY720, an S1P modulator. NIK-mutated stromal cells showed aberrant expression of VCAM-1, ICAM-1, and CXCL13, leading to the impaired ability to support S1P-mediated peritoneal B cell emigration. Therefore, aly peritoneal B cells exhibited normal S1P-mediated peritoneal B cell trafficking from peritoneum to intestine for IgA production when they were adoptively transferred into SCID mice. Further, transfer of wild-type stromal cells into the peritoneum restored S1P-mediated trafficking of aly peritoneal B cells. These findings suggest that NIK in stromal cells has a specific role in the regulation of S1P-mediated peritoneal B cell-derived intestinal IgA production. This work was supported by grants from the Ministry of Education, Science, Sports, and Culture of Japan and the Ministry of Health and Welfare of Japan.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.182.Supp.39.9
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2009
detail.hit.zdb_id:
1475085-5
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