In:
Genome Research, Cold Spring Harbor Laboratory, Vol. 18, No. 7 ( 2008-07), p. 1064-1072
Abstract:
A key element for defining the centromere identity is the incorporation of a specific histone H3, CENPA, known as Cnp1p in Schizosaccharomyces pombe . Previous studies have suggested that functional S. pombe centromeres lack regularly positioned nucleosomes and may involve chromatin remodeling as a key step of kinetochore assembly. We used tiling microarrays to show that nucleosomes are, in fact, positioned in regular intervals in the core of centromere 2, providing the first high-resolution map of regional centromere chromatin. Nucleosome locations are not disrupted by mutations in kinetochore protein genes cnp1 , mis18 , mis12 , nuf2 , mal2 ; overexpression of cnp1 ; or the deletion of ams2 , which encodes a GATA-like factor participating in CENPA incorporation. Bioinformatics analysis of the centromere sequence indicates certain enriched motifs in linker regions between nucleosomes and reveals a sequence bias in nucleosome positioning. In addition, sequence analysis of nucleosome-free regions identifies novel binding sites of Ams2p. We conclude that centromeric nucleosome positions are stable and may be derived from the underlying DNA sequence.
Type of Medium:
Online Resource
ISSN:
1088-9051
DOI:
10.1101/gr.075374.107
Language:
English
Publisher:
Cold Spring Harbor Laboratory
Publication Date:
2008
detail.hit.zdb_id:
1483456-X
SSG:
12
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