In:
Molecular Nutrition & Food Research, Wiley, Vol. 58, No. 4 ( 2014-04), p. 782-798
Abstract:
The major alimentary sources for the plasma membrane lipid sphingomyelin ( SM ) are dairy products, eggs, and meat. We recently reported that the SM metabolite ceramide induces cathepsin D mediated apoptosis in murine intestinal epithelial cells ( IEC s) and increases inflammation in acute colitis. We investigated the impact of SM and phosphatidylcholine on apoptosis in human IEC s and point out BH 3‐interacting death agonist ( BID ) as link between cathepsin D and apoptosis. Methods and results HT ‑29 and isolated human IEC s were stimulated with SM or phosphatidylcholine. SM treatment resulted in increased apoptosis. Phosphatidylcholine showed contrary effects. W estern revealed higher amounts of cathepsin D and BID activation upon lipid stimulation. W estern blotting revealed BID activation through SM in both an induced and a spontaneous mouse model of colitis. Conclusion Dietary phospholipids may induce or abolish apoptosis in IEC s and seem to play a role in the pathogenesis of inflammatory bowel diseases. This nutritional factor might be considered when evaluating the pathogenesis of inflammatory bowel diseases. Effects of SM ase‐ and SM treatment on inflammation in dextran sulfate sodium induced animal models of colitis and in vitro experiments are discussed as controversial. Variable sources of SM , feeding techniques, and mouse strains might play a role.
Type of Medium:
Online Resource
ISSN:
1613-4125
,
1613-4133
DOI:
10.1002/mnfr.201300369
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2160372-8
SSG:
12
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