In:
Nephron, S. Karger AG, Vol. 90, No. 3 ( 2002), p. 313-319
Abstract:
〈 i 〉 Background/Aims: 〈 /i 〉 Quercetin, a naturally occurring dietary flavonoid, is a potent in vitro inhibitor of the transcription factors, nuclear factor (NF)-ĸB and activator protein (AP)-1. Here we investigated the efficacy of quercetin to suppress renal cortical NF-ĸB/AP-1 activation and tubulointerstitial injury, in vivo, in a nephrotic rat model of chronic glomerular disease. 〈 i 〉 Methods: 〈 /i 〉 Adult male Wistar rats with (n = 27) or without (n = 15) Adriamycin nephropathy (AN) were stratified into six groups (according to proteinuria and endogenous creatinine clearance, measured on day 10) and pair-fed a semipurified diet supplemented with or without quercetin (0.5, 2%) from day 14 to 36. 〈 i 〉 Results: 〈 /i 〉 Quercetin-fed rats had minimal weight gain during the study (p 〈 0.05). In AN, proteinuria, the decline in endogenous creatinine clearance, hypercholesterolaemia and cortical tubulointerstitial injury were not reduced by quercetin. Quercetin had no effect on renal cortical NF-ĸB activation, but unexpectedly exacerbated the induction of AP-1 in AN (by 67%, p 〈 0.05). In normal rats, quercetin increased proteinuria (by 75%, p 〈 0.05), renal cortical AP-1 activation (by 198%, p = 0.06), and malondialdehyde production (by 110%, p 〈 0.05). 〈 i 〉 Conclusion: 〈 /i 〉 These data suggest that supplementation of the diet with quercetin is not an effective therapeutic strategy alone to reduce NF-ĸB/ AP-1 activation in chronic glomerular diseases, and paradoxically could be associated with adverse renal effects.
Type of Medium:
Online Resource
ISSN:
1660-8151
,
2235-3186
Language:
English
Publisher:
S. Karger AG
Publication Date:
2002
detail.hit.zdb_id:
2810853-X
Permalink