In:
British Journal of Nutrition, Cambridge University Press (CUP), Vol. 109, No. 8 ( 2013-04-28), p. 1349-1358
Abstract:
Muscle atrophy increases the production of reactive oxygen species and the expression of atrophy-related genes, which are involved in the ubiquitin–proteasome system. In the present study, we investigated the effects of β-carotene on oxidative stress (100 μ m -H 2 O 2 )-induced muscle atrophy in murine C2C12 myotubes. β-Carotene (10 μ m ) restored the H 2 O 2 -induced decreased levels of myosin heavy chain and tropomyosin ( P 〈 0·05, n 3) and decreased the H 2 O 2 -induced increased levels of ubiquitin conjugates. β-Carotene reduced the H 2 O 2 -induced increased expression levels of E3 ubiquitin ligases (Atrogin-1 and MuRF1) and deubiquitinating enzymes (USP14 and USP19) ( P 〈 0·05, n 3) and attenuated the H 2 O 2 -induced nuclear localisation of FOXO3a. Furthermore, we determined the effects of β-carotene on denervation-induced muscle atrophy. Male ddY mice (8 weeks old, n 30) were divided into two groups and orally pre-administered micelle with or without β-carotene (0·5 mg once daily) for 2 weeks, followed by denervation in the right hindlimb. β-Carotene was further administered once daily until the end of the experiment. At day 3 after denervation, the ratio of soleus muscle mass in the denervated leg to that in the sham leg was significantly higher in β-carotene-administered mice than in control vehicle-administered ones ( P 〈 0·05, n 5). In the denervated soleus muscle, β-carotene administration significantly decreased the expression levels of Atrogin-1, MuRF1, USP14 and USP19 ( P 〈 0·05, n 5) and the levels of ubiquitin conjugates. These results indicate that β-carotene attenuates soleus muscle loss, perhaps by repressing the expressions of Atrogin-1, MuRF1, USP14 and USP19, at the early stage of soleus muscle atrophy.
Type of Medium:
Online Resource
ISSN:
0007-1145
,
1475-2662
DOI:
10.1017/S0007114512003297
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2013
detail.hit.zdb_id:
2016047-1
SSG:
12
SSG:
21
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