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  • Hanley, Thomas R.  (2)
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  • 1
    Online Resource
    Online Resource
    Magnetic particle characterization—magnetophoretic mobility and particle size
    Wiley ; 2016
    In:  Cytometry Part A Vol. 89, No. 6 ( 2016-06), p. 585-593
    Details
    In: Cytometry Part A, Wiley, Vol. 89, No. 6 ( 2016-06), p. 585-593
    Abstract: Quantitative characterization of magnetic particles is useful for analysis and separation of labeled cells and magnetic particles. A particle velocimeter is used to directly measure the magnetophoretic mobility, size, and other parameters of magnetic particle suspensions. The instrument provides quantitative video analysis of particles and their motion. The trajectories of magnetic particles in an isodynamic magnetic field are recorded using a high‐definition camera/microscope system for image collection. Image analysis software then converts the image data to the parameters of interest. The distribution of magnetophoretic mobility is determined by combining fast image analysis with velocimetry measurements. Particle size distributions have been characterized to provide a better understanding of sample quality. The results have been used in the development and operation of analyzer protocols for counting particle concentrations accurately and measuring magnetic susceptibility and size for simultaneous display for routine application to particle suspensions and magnetically labeled biological cells. © 2016 International Society for Advancement of Cytometry
    Type of Medium: Online Resource
    ISSN: 1552-4922 , 1552-4930
    URL: Issue
    URL: Article
    DOI: 10.1002/cyto.a.v89.6
    DOI: 10.1002/cyto.a.22866
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2180639-1
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Continuous Flow Labeling and In-Line Magnetic Separation of Cells
    MDPI AG ; 2021
    In:  Magnetochemistry Vol. 8, No. 1 ( 2021-12-30), p. 5-
    Details
    In: Magnetochemistry, MDPI AG, Vol. 8, No. 1 ( 2021-12-30), p. 5-
    Abstract: There is an identified need for point-of-care diagnostic systems for detecting and counting specific rare types of circulating cells in blood. By adequately labeling such cells with immunomagnetic beads and quantum dots, they can be efficiently collected magnetically for quantification using fluorescence methods. Automation of this process requires adequate mixing of the labeling materials with blood samples. A static mixing device can be employed to improve cell labeling efficiency and eliminate error-prone laboratory operations. Computational fluid dynamics (CFD) were utilized to simulate the flow of a labeling-materials/blood mixture through a 20-stage in-line static mixer of the interfacial-surface-generator type. Optimal fluid mixing conditions were identified and tested in a magnetic bead/tumor cell model, and it was found that labeled cells could be produced at 1.0 mL/min flow rate and fed directly into an in-line magnetic trap. The trap design consists of a dual flow channel with three bends and a permanent magnet positioned at the outer curve of each bend. The capture of labeled cells in the device was simulated using CFD, finite-element analysis and magnetophoretic mobility distributions of labeled cells. Testing with cultured CRL14777 human melanoma cells labeled with anti-CD146 1.5 μm diameter beads indicated that 90 ± 10% are captured at the first stage, and these cells can be captured when present in whole blood. Both in-line devices were demonstrated to function separately and together as predicted.
    Type of Medium: Online Resource
    ISSN: 2312-7481
    URL: Article
    DOI: 10.3390/magnetochemistry8010005
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2840617-5
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