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  • 1
    Online Resource
    Online Resource
    AGROBACTERIUM-MEDIATED TRANSFORMATION OF PANAX VIETNAMENSIS HA ET GRUSHV.
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 15, No. 4 ( 2018-12-14), p. 641-650
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 15, No. 4 ( 2018-12-14), p. 641-650
    Abstract: In recent years, the Agrobacterium-mediated genetic transformation system has become the most useful method widely used for the introduction of foreign genes into plant cells followed by regeneration of genetically improved plants. Panax vietnamensis Ha et Grushv. is a highly valued medicinal plant native to Vietnam with limited area of distribution. This report illustrates the possibilities of biotechnology for genetic transformation aimed at establishing an effective production of secondary metabolites in P. vietnamensis. In the present investigation, 0.5 cm2 leaf blades, 1 cm long leaf petioles and 0.5 cm3 callus clusters were used for the hairy root induction. Results indicated that hairy roots were induced on P. vietnamensis callus clusters co-cultivated with Agrobacterium rhizogenes strain ATCC15834 at OD600 of 0.5 with an infection time of 20 min and a supplementation of 100 mM acetosyringone. PCR amplification of the DNA isolated from the resulting hairy roots was used to confirm the presence of rol genes. Compared to in vitro rhizome cultures, hairy root cultures appear to be potential for continuous production of valuable secondary metabolites with similar saponin profiles. The protocol described in this study is simple and rapid and therefore, can be used for large-scale experiments for the rapid production of valuable compounds.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15/4/13404
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 2
    Online Resource
    Online Resource
    Efficient transgenic plantlet regeneration from hairy roots via somatic embryogenesis and hardening plantlets of Panax vietnamensis by iron nanoparticles-supplied culture
    Springer Science and Business Media LLC ; 2022
    In:  Plant Cell, Tissue and Organ Culture (PCTOC) Vol. 151, No. 2 ( 2022-11), p. 335-345
    Details
    In: Plant Cell, Tissue and Organ Culture (PCTOC), Springer Science and Business Media LLC, Vol. 151, No. 2 ( 2022-11), p. 335-345
    Type of Medium: Online Resource
    ISSN: 0167-6857 , 1573-5044
    URL: Article
    DOI: 10.1007/s11240-022-02355-9
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 1478391-5
    SSG: 12
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  • 3
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    Online Resource
    LED Lights Promote Growth and Flavonoid Accumulation of Anoectochilus roxburghii and Are Linked to the Enhanced Expression of Several Related Genes
    MDPI AG ; 2020
    In:  Plants Vol. 9, No. 10 ( 2020-10-12), p. 1344-
    Details
    In: Plants, MDPI AG, Vol. 9, No. 10 ( 2020-10-12), p. 1344-
    Abstract: Anoectochilus roxburghii is a wild edible species and has been traditionally used for a wide range of diseases in many countries. Our research aims to find the optimal light-emitting diode (LED) lighting conditions to improve the growth and development of A. roxburghii seedling at the acclimation stage. Two-month-old explants were cultured under the various lighting conditions including red (R), blue (B), BR (one blue: four red), BRW151 (one blue: five red: one white), BRW142 (one blue: four red: two white), and fluorescent lamp (FL). The results showed that the lighting conditions not only affect the growth and morphology of plants but also the accumulation of total flavonoids. Single wavelengths (B or R LED) inhibited the growth and secondary biosynthesis of A. roxburghii, while the BR LED showed an enhancement in both growth and biomass accumulation. A. roxburghii plants were grown under BR LED light has average plant height (7.18 cm), stem diameter (17.6mm), number of leaves (5.78 leaves/tree), leaf area (4.67 cm2), fresh weight (0.459 g/tree), dry matter percentages (11.69%), and total flavonoid (1.811 mg/g FW) is considered to be superior to FL lamps and other LEDs in the experiment. This indicates that both blue and red wavelengths are required for the normal growth of A. roxburghii. To learn more about how light affects flavonoid biosynthesis, we evaluated the expression of genes involved in this process (pal, chs, chi, and fls) and found that BR LED light enhances the expression level of chi and fls genes compared to fluorescent lamps (1.18 and 1.21 times, respectively), leading to an increase in the flavonoid content of plant. Therefore, applying BR LED during in vitro propagation of A. roxburghii could be a feasible way to improve the medicinal value of this plant.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    URL: Article
    DOI: 10.3390/plants9101344
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2704341-1
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  • 4
    Online Resource
    Online Resource
    Study on the effects of led light on physiological and morphological characteristics of dang shen (Codonopsis sp.) growth in in vitro condition
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2016
    In:  TAP CHI SINH HOC Vol. 38, No. 2 ( 2016-05-30)
    Details
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 38, No. 2 ( 2016-05-30)
    Type of Medium: Online Resource
    ISSN: 0866-7160 , 0866-7160
    URL: Article
    DOI: 10.15625/0866-7160/v38n2.7106
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2016
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  • 5
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    Online Resource
    Comparison of morphogenesis and anatomy between hairy roots and adventitious roots of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.)
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Biotechnology Vol. 17, No. 3 ( 2020-11-28), p. 483-490
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 17, No. 3 ( 2020-11-28), p. 483-490
    Abstract: In this study, morphogenesis and anatomy of adventitious roots and hairy roots of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) were compared. Adventitious roots were derived from four different samples (leaf, petiole, root and callus) in vitro cultured on medium SH supplemented with 5 mg/L IBA. Hairy roots were derived from callus infected with Agrobacterium rhizogenes strain ATCC 15834. The results showed that there were significant differences in morphogenesis and anatomy, the pathway of secondary roots formation, culture medium between adventitous roots and hairy roots. The hairy roots strongly grew and branched on the free-plant growth regulator medium. The secondary root of the hairy root was formed in two pathways: (1) The secondary root formation began at the vesicles system of main root; (2) The secondary root formation was independent of the vesicles system of main root; it kept developing in length and connected to the vesicles system of main root. On the other hand, growing adventitous roots only grew on the environment with exogenous auxin supplementation; also their branching ability were low. The secondary root formation of the adventitous root was started at the vesicles of the main roots and there was only one secondary root that was formed at an location of adventitous root. The results that obtained in this study will be the reference to identify two types of root.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/17/3/10886
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2020
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  • 6
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    Online Resource
    Isolation and screening of endophytic bacteria from Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv) for biosynthesis β-glucosidase
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  TAP CHI SINH HOC Vol. 40, No. 2 ( 2018-11-06), p. 153-161
    Details
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 40, No. 2 ( 2018-11-06), p. 153-161
    Abstract: Biotransformation of secondary metabolites from medicinal plants by bacteria has been studied extensively in the recent years. One of the potential sources for screening bacteria is plant endophytic bacteria. In this study, we isolated and screened endophytic bacteria for transformation of ginsenoside Rb1 of Ngoc Linh ginseng collected at village number 2, Tra Linh commune, Nam Tra My district, Quang Nam province. There are 45 strains isolated form rhizobium (24 strains), petioles (8 strains) and leaves (13 strains). After screening, there are 27 strains positive with β-glucosidase test, an enzyme which catalyses the hydrolysis of terminal non-reducing residues in β-glucosides-aglycone linkage of ginsenoside Rb. By evaluating the β-glucosidase activity and identification via 16S rRNA sequence, we choosed four high β-glucosidase acitivity strains SVK32 (Enterobacter sp.); SVK34 (Serratia sp.); SVK37 (Ochrobactrum sp.) and SVK44 (Arthrobacter sp.) for futher study on biotransformating of ginsenoside Rb1 into ginsenoside Rd and Rg3.
    Type of Medium: Online Resource
    ISSN: 0866-7160 , 0866-7160
    URL: Article
    DOI: 10.15625/0866-7160/v40n2.10862
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 7
    Online Resource
    Online Resource
    Effects of light emitting diodes - led on regeneration ability of Coffea canephora mediated via somatic embryogenesis
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2016
    In:  TAP CHI SINH HOC Vol. 38, No. 2 ( 2016-05-30)
    Details
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 38, No. 2 ( 2016-05-30)
    Type of Medium: Online Resource
    ISSN: 0866-7160 , 0866-7160
    URL: Article
    DOI: 10.15625/0866-7160/v38n2.7115
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2016
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  • 8
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    Online Resource
    OPTIMIZATION OF EXPRESSION CONDITIONS OF GENE ENCODING ANTIGEN M OF PRRSV IN LEAVES OF NICOTIANA BENTHAMIANA BY AGROINFILTRATION METHOD
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 16, No. 2 ( 2018-12-17), p. 293-300
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 16, No. 2 ( 2018-12-17), p. 293-300
    Abstract: Reproductive disorders and respiratory swine (Porcine Reproductive and Respiratory Syndrome - PRRS) is an infectious disease caused by the PRRS virus (PRRSV), spread fast speeds, causing mass death when infected pigs. M protein is one of the major structural protein of the PRRSV, has a molecular weight of about 19 kDa, encoded by the open reading frame 6 (ORF6), which is used to design a subunit vaccine against back PRRSV. In this study, conditions for transient expression of the gene encoded the M protein of PRRSV in leaves of the Nicotiana benthamiana by agroinfiltration method were determined. The results show that optimal conditions for transient expression of protein M in the leaf are used the simultaneous of the A. tumefaciens strain containing vector carrying the gene encoding the protein M and A. tumefaciens containing vector carrying gene encoding supported protein HC-Pro PVY, concentration of acetosyringone with 450 µM, the bacterial cell density used to infect into leaf with OD600 is 0.5, The physiological age of the leaf suitable for infecting bacteria was young leaf and mature leaf of tobacco plants 4 to 6 weeks of age, and the transient expression time of gene encoding protein M of PRRSV in tobacco leaves is most effective 6 days after infecting. This agroinfiltration method can be used to express large amounts of protein - antigen M of PRRSV in tobacco plants to produce vaccines against this virus.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/16/2/13440
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 9
    Online Resource
    Online Resource
    Gene expression profiling of adp-glucose pyrophosphorylase (AGPase) in sink and source organs of some cassava varieties with different starch contents in Vietnam
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 14, No. 4 ( 2018-04-19), p. 673-682
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 14, No. 4 ( 2018-04-19), p. 673-682
    Abstract: Starch is the most widespread and abundant storage carbohydrate in plants. We depend upon starch for our nutrition, exploit its unique properties in industry, and use it as a feedstock for bio-ethanol production. Starch is stored in the form of osmotically inactive, water-insoluble granules in amyloplasts (storage starch) and chloroplasts (transitory starch). The biosynthesis of starch involves not only the production of the composite glucans but also their arrangement into an organized form within the starch granule. Understanding the specific functions played by individual isoforms of enzymes involved in starch biosynthesis pathways will provide important basis for regulation of starch production in plant. A transcript-level analysis of the genes which encode starch-synthesis enzymes is fundamental for assessment of enzyme function and the regulatory mechanism for starch biosynthesis in source and sink organs. In this work, the expression level of the genes encoding ADP-glucose pyrophosphorylase (AGPase) in two local varieties Do Dia Phuong (Do DF) and Trang Hoa Binh (Trang HB) as well as two imported varieties KM94 (Rayong1 X Rayong 90) and KM140 (KM98-1 x KM36) with different starch contents were evaluated by quantitative real-time PCR method. The result of transcript level analysis made the expression profiles of cassava AGPS and AGPL genes (encoding AGPase small and large subunits) during three development periods, 90, 180 and 270 DAP (day after planting). The transcriptional activities of these genes exhibited tissue-specific expression patterns. In particular, AGPS2 and AGPL1 transcripts were predominant in leaves, whereas expression of AGPS1, AGPL2, and AGPL3 appeared to be mostly confined to storage roots. Despite of having disparities between development stages, expression patterns of both AGPS2 and AGPL1 in leaves did not show significant differences amongst investigated cassava varieties. In contrast, transcriptional activities of AGPS1 and AGPL3 in tubers had patterns directly related to the starch contents of the cultivars. These results indicated that AGPS1 and AGPL3 genes likely play an important role in the starch biosynthesis pathway and have potential for regulation of starch production in cassava.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/14/4/12300
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 10
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    Online Resource
    Studying on regeneration of several cassava varieties (Manihot esculenta Crantz) via friable embryogenic callus
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 16, No. 1 ( 2018-12-17), p. 119-126
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 16, No. 1 ( 2018-12-17), p. 119-126
    Abstract: Cassava (Manihotesculenta Crantz) is considered as one of the most important food crops which has high economic value in many areas. It is very neccessay to perfect the cassava regeneration protocol for genetic transformation purpose. In this study, cassava regeneration via friable embryogenic callus (FEC) from tip bud, young stem, pieces of leaf had been optimized in five cassava varieties which were planted in Vietnam including KM 140 (S1), NgheAn white cassava (S2), Lang Son red cassava (S3), HoaBinh high – yield cassava (S4) and Huay Bong (S5). The results indicated that on MS medium supplemented with 10 mg/l Picloram, the proportion of callus formation was very high, reached from 90 to 100%.  In the case of using tip buds, after three weeks, calli were transferred to MS medium adding 5 mg/l picloram and 0,2 mg/l IBA. The proportion of FEC formation reached 41,1 – 80,4 % after 8 weeks of cultivation in all studied Cassava varieties. The samples were transferred to MS medium adding 0,3 mg/l BAP to elongate shoots in 4 weeks. The highest regeneration rate belonged to S1, and was 61,67%. Three weeks after shoot transferring on MS medium, the complete seedlings were grown in substrate which was composed by TN01 and husk hun with ratio of 6:4 in greenhouse. As a result, the rate of survival plants reached to 95%. The process of regeneration of cassava through embryonic calli could be applied for the improvement of desired cassava varieties by method of genetic engineering.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/16/1/13456
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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