In:
European Journal of Biochemistry, Wiley, Vol. 137, No. 1-2 ( 1983-12), p. 257-262
Abstract:
Poly(A)‐rich RNA was isolated from the livers of acutely inflamed rats by extraction with guanidinium HCl and oligo(dT)‐cellulose chromatography. After translation in a recticulocyte lysate and immunoprecipitation with a specific antiserum to α2‐macroglobulin a polypeptide with an apparent molecular weight of 162000 could be detected. The cell‐free synthesis of α2‐macroglobulin was stimulated 8‐fold by the addition of RNase inhibitor. Full‐length α2‐macroglobulin polypeptide chains appeared after 35 min in the presence of 1.85 mM Mg 2+ and 100 mM K + . A nucleotide number of about 5100 was estimated for α2‐macroglobulin by means of sucrose gradient centrifugation of poly(A)‐rich RNA followed by translation in vitro and immunoprecipitation of α2‐macroglobulin. In normal liver α2‐macroglobulin mRNA represented about 0.0007% of total translatable RNA. Acute inflammation generated by intramuscular injection of turpentine led to a 66‐fold increase in translatable α2‐macroglobulin mRNA after 18 h, followed by a rapid decrease. In accordance to the induction of α2‐macroglobulin mRNA serum concentrations of α2‐macroglobulin increased to about 2 mg/ml. Unlike α2‐macroglobulin mRNA serum α2‐macroglobulin levels remained unchanged up to 60 h.
Type of Medium:
Online Resource
ISSN:
0014-2956
,
1432-1033
DOI:
10.1111/ejb.1983.137.issue-1-2
DOI:
10.1111/j.1432-1033.1983.tb07823.x
Language:
English
Publisher:
Wiley
Publication Date:
1983
detail.hit.zdb_id:
1398347-7
detail.hit.zdb_id:
2172518-4
SSG:
12
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