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  • HABURA, ANDREA  (2)
  • 2000-2004  (2)
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  • 2000-2004  (2)
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  • 1
    Online Resource
    Online Resource
    Wiley ; 2004
    In:  Journal of Eukaryotic Microbiology Vol. 51, No. 2 ( 2004-03), p. 173-179
    In: Journal of Eukaryotic Microbiology, Wiley, Vol. 51, No. 2 ( 2004-03), p. 173-179
    Abstract: ABSTRACT. Studies of benthic Foraminifera typically rely on the morphological identification of dried specimens. This approach can introduce sampling bias against small, delicate, or morphologically ambiguous forms. To overcome this limitation, we extracted total DNA from sediment followed by PCR using group‐ and species‐specific primers. Phylogenetic analyses revealed that approximately ninety percent of the PCR products represented previously undescribed sequence types that group with undersampled members of the allogromiid Foraminifera. We also used a modification of this technique to track individual species in sediment fractions too fine for normal morphological identification, and to confirm species placement of morphologically ambiguous foraminiferans. We were able to identify the DNA of several large foraminiferal species in fine fractions in a seasonally‐dependent manner, indicating that in some seasons the majority of the standing stock of these species exists as gametes/juveniles. The approach outlined here represents a powerful strategy for exploring the total diversity of benthic foraminiferal communities.
    Type of Medium: Online Resource
    ISSN: 1066-5234 , 1550-7408
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2004
    detail.hit.zdb_id: 2126326-7
    SSG: 12
    Location Call Number Limitation Availability
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  • 2
    Online Resource
    Online Resource
    Wiley ; 2003
    In:  Journal of Eukaryotic Microbiology Vol. 50, No. 2 ( 2003-03), p. 135-139
    In: Journal of Eukaryotic Microbiology, Wiley, Vol. 50, No. 2 ( 2003-03), p. 135-139
    Abstract: ABSTRACT. Sediment‐dwelling protists are among the most abundant meiobenthic organisms, ubiquitous in all types of aquatic ecosystems. Yet, because their isolation and identification are difficult, their diversity remains largely unknown. In the present work, we applied molecular methods to examine the diversity of freshwater Foraminifera, a group of granuloreticulosan protists largely neglected until now. By using specific PCR primers, we detected the presence of Foraminifera in all sediment samples examined. Phylogenetic analysis of amplified SSU rDNA sequences revealed two distinct groups of freshwater foraminiferans. All obtained sequences branched within monothalamous (single‐chambered), marine Foraminifera, suggesting a repeated colonization of freshwater environments. The results of our study challenge the traditional view of Foraminifera as essentially marine organisms, and provide a conceptual framework for charting the molecular diversity of freshwater granuloreticulosan protists.
    Type of Medium: Online Resource
    ISSN: 1066-5234 , 1550-7408
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2003
    detail.hit.zdb_id: 2126326-7
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
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