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  • Guo, Chaowan  (4)
  • 1
    Online Resource
    Online Resource
    Enhanced exopolysaccharide yield and antioxidant activities of Schizophyllum commune fermented products by the addition of Radix Puerariae
    Royal Society of Chemistry (RSC) ; 2021
    In:  RSC Advances Vol. 11, No. 60 ( 2021), p. 38219-38234
    Details
    In: RSC Advances, Royal Society of Chemistry (RSC), Vol. 11, No. 60 ( 2021), p. 38219-38234
    Abstract: To increase the production of exopolysaccharides (EPS) and expand the application of Schizophyllum commune ( S. commune ) fermentation liquid, the traditional Chinese medicine Radix Puerariae (RP) with outstanding biological activity was selected as a culture additive to improve the EPS yield and enhance the antioxidant activity of fermented products from S. commune . The effects of three independent factors: A: initial pH (5.0–6.0), B: concentration of RP (10–14 g L −1 ), and C: inoculum size (8–12%, v/v) on the EPS yield were evaluated. The results of response surface methodology (RSM) showed that the optimal fermentation conditions were: A: 5.40, B: 12.80 g L −1 , and C: 10.0%. The optimal yield of EPS was 8.41 ± 0.12 mg mL −1 , which showed an insignificant ( p 〉 0.05) difference with the predicted value (8.45 mg mL −1 ). The fermented supernatants cultured from RP-supplemented medium (SC-RP) or regular medium (SC) were collected for further study. FT-IR analysis of EPS-1 (purified from SC) and EPS-2 (purified from SC-RP) showed that their structures were consistent, indicating that the addition of RP did not affect the structure of schizophyllan (SPG). In addition, compared with SC, the in vitro antioxidant activities of SC-RP were significantly improved with ORAC values and FRAP values increasing by 11.56-fold and 14.69-fold, respectively. There was a significant correlation among the phenolic compounds, flavonoids, and antioxidant activity of SC-RP in this study. Besides, SC-RP was detected to contain more than 25 bioactive ingredients compared with that of SC, which may play a key role in its antioxidant activities. Thus, these results indicated that RP enhanced the yield of SPG and improved the antioxidant activity of the fermented products by S. commune . Accordingly, the fermentation liquid of S. commune with the addition of RP may have potential application in food, cosmetics, and pharmaceutical industries.
    Type of Medium: Online Resource
    ISSN: 2046-2069
    URL: Article
    DOI: 10.1039/D1RA06314F
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2021
    detail.hit.zdb_id: 2623224-8
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  • 2
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    Table_1.pdf
    Frontiers Media SA ; 2022
    In:  Frontiers in Nutrition Vol. 9 ( 2022-3-11)
    Details
    In: Frontiers in Nutrition, Frontiers Media SA, Vol. 9 ( 2022-3-11)
    Abstract: Schizophyllum commune ( S. commune ) fermented supernatant with added Radix Puerariae (SC-RP) showed significant antioxidant activity in our previous work. However, the possible lifespan and healthspan extending the capacity of Caenorhabditis elegans ( C. elegans ) and the underlying mechanism were not illuminated. In this study, the effect of SC-RP on extending the lifespan and improving stress resistance of C. elegans were examined. Additionally, the underlying lifespan extending molecular mechanisms of SC-RP were explored. Treated with SC-RP at 10 μg/mL, the lifespan of C. elegans increased by 24.89% ( P & lt; 0.01). Also, SC-RP prolonged the healthspan of the nematode, including reducing lipofuscin levels, improving mobility and enhancing resistance to oxidative stress and heat shock. Moreover, superoxide dismutase and catalase activities were increased for SC-RP treated C. elegans . Meantime the intracellular levels of thiobarbituric acid reactive substances (TBARS) and reactive oxygen species (ROS) were attenuated. Express levels of eight genes including daf-2, daf-16, sod-3, skn-1, gst-4, clk-1, age-1 and mev-1 were analyzed by RT-PCR method for possible C. elegan anti-aging mechanisms of SC-RP. Expression levels of key genes daf-2, gst-4 and sod-3 were up-regulated, while that of daf-16, skn-1 , and clk-1 were down-regulated. The results suggest that SC-RP could extend the lifespan and healthspan of C. elegans significantly, and the IIS pathway, SKN-1/Nrf2 pathway and mitochondrial metabolism pathway were primarily considered associated. Thus, SC-RP is a potential component to improve aging and aging-related symptoms as new functional materials.
    Type of Medium: Online Resource
    ISSN: 2296-861X
    URL: Article
    URL: Article
    DOI: 10.3389/fnut.2022.847064
    DOI: 10.3389/fnut.2022.847064.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2776676-7
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  • 3
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    High-voltage pulse-assisted extraction of flavonoids from kapok using deep eutectic solvent aqueous solutions
    Royal Society of Chemistry (RSC) ; 2022
    In:  RSC Advances Vol. 12, No. 38 ( 2022), p. 25025-25034
    Details
    In: RSC Advances, Royal Society of Chemistry (RSC), Vol. 12, No. 38 ( 2022), p. 25025-25034
    Abstract: In this study, deep eutectic solvents coupled with a pulsed electric field (PEF-DES) were first applied to the extraction of traditional Chinese medicine plants. This study uses the PEF-DES extraction technique to extract TG-KF (Kapok flavonoid solution extracted with DES-TG). PEF-DES is a simple, effective and environmentally-friendly technology and can be used in industrial-scale production. For the optimal extraction conditions of TG-KF, DES-TG was used as a solvent, the DES-TG concentration was 50%, the solid–liquid ratio was 1 : 30, the electric field intensity was 0.55 kV cm −1 , the number of pulses was 100, and the yield of flavonoids was 14.36 ± 0.35%. TG-KF has very good stability and there is no precipitation or discoloration within 6 months. The results of chicken embryo experiments and human patch tests show that 10% TG-KF aqueous solution has no irritation. DPPH experiments show that TG-KF has excellent efficacy as an antioxidant. Overall, TG-KF is expected to become a potential antioxidant raw material.
    Type of Medium: Online Resource
    ISSN: 2046-2069
    URL: Article
    DOI: 10.1039/D2RA03969A
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2022
    detail.hit.zdb_id: 2623224-8
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  • 4
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    Production and Anti-Inflammatory Performance of PVA Hydrogels Loaded with Curcumin Encapsulated in Octenyl Succinic Anhydride Modified Schizophyllan as Wound Dressings
    MDPI AG ; 2023
    In:  Molecules Vol. 28, No. 3 ( 2023-01-30), p. 1321-
    Details
    In: Molecules, MDPI AG, Vol. 28, No. 3 ( 2023-01-30), p. 1321-
    Abstract: Amphiphilic polysaccharides can be used as wall materials and applied to encapsulate hydrophobic active chemicals; moreover, there is significant demand for novel medical high-molecular-weight materials with various functions. In order to prepare amphiphilic schizophyllan (SPG), octenyl succinic anhydride (OSA) was chosen to synthesize OSA-modified schizophyllan (OSSPG) using an esterified reaction. The modification of OSSPG was demonstrated through FT-IR and thermal analysis. Moreover, it was found that OSSPG has a better capacity for loading curcumin, and the loading amount was 20 μg/mg, which was 2.6 times higher than that of SPG. In addition, a hydrogel made up of PVA, borax, and C-OSSPG (OSSPG loaded with curcumin) was prepared by means of the one-pot method, based on the biological effects of curcumin and the immune-activating properties of SPG. The mechanical properties and biological activity of the hydrogel were investigated. The experimental results show that the dynamic cross-linking of PVA and borax provided the C-OSSPG/BP hydrogel dressing with exceptional self-healing properties, and it was discovered that the C-OSSPG content increased the hydrogel’s swelling and moisturizing properties. In fibroblast cell tests, the cells treated with hydrogel had survival rates of 80% or above. Furthermore, a hydrogel containing C-OSSPG could effectively promote cell migration. Due to the excellent anti-inflammatory properties of curcumin, the hydrogel also significantly reduces the generation of inflammatory factors, such as TNF-α and IL-6, and thus has a potential application as a wound dressing medicinal material.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    URL: Article
    DOI: 10.3390/molecules28031321
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2008644-1
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