In:
Frontiers in Neuroscience, Frontiers Media SA, Vol. 15 ( 2022-3-3)
Abstract:
Electrophysiological recordings lead amongst the techniques that aim to investigate the dynamics of neural activity sampled from large neural ensembles. However, the financial costs associated with the state-of-the-art technology used to manufacture probes and multi-channel recording systems make these experiments virtually inaccessible to small laboratories, especially if located in developing countries. Here, we describe a new method for implanting several tungsten electrode arrays, widely distributed over the brain. Moreover, we designed a headstage system, using the Intan ® RHD2000 chipset, associated with a connector (replacing the expensive commercial Omnetics connector), that allows the usage of disposable and inexpensive cranial implants. Our results showed high-quality multichannel recording in freely moving animals (detecting local field, evoked responses and unit activities) and robust mechanical connections ensuring long-term continuous recordings. Our project represents an open source and inexpensive alternative to develop customized extracellular records from multiple brain regions.
Type of Medium:
Online Resource
ISSN:
1662-453X
DOI:
10.3389/fnins.2021.691788
DOI:
10.3389/fnins.2021.691788.s001
DOI:
10.3389/fnins.2021.691788.s002
DOI:
10.3389/fnins.2021.691788.s003
DOI:
10.3389/fnins.2021.691788.s004
DOI:
10.3389/fnins.2021.691788.s005
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2411902-7
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