In:
Development, Growth & Differentiation, Wiley, Vol. 56, No. 9 ( 2014-12), p. 615-624
Abstract:
Insulin‐like growth factor 1 ( IGF ‐1) is a multifunctional peptide that can enhance osteogenic differentiation of bone marrow mesenchymal stem cells ( BMMSC s). However, it remains unclear whether IGF ‐1 can promote osteogenic differentiation of human dental pulp stem cells ( DPSC s). In our study, DPSC s were isolated from the impacted third molars, and treated with IGF ‐1. Osteogenic differentiation abilities were investigated. We found that IGF ‐1 activated the m TOR signaling pathway during osteogenic differentiation of DPSC s. IGF ‐1 also increased the expression of runt‐related transcription factor 2 ( RUNX 2), osteocalcin ( OCN ), osterix ( OSX ) and collagen type I ( COL I ) during this process. Rapamycin, an m TOR inhibitor, blocked osteogenic differentiation induced by IGF ‐1. Meanwhile, CCK ‐8 assay and flow cytometry results demonstrated that 10–200 ng/mL IGF ‐1 could enhance proliferation ability of DPSC s and 100 ng/mL was the optimal concentration. In summary, IGF ‐1 could promote proliferation and osteogenic differentiation of DPSC s via m TOR pathways, which might have clinical implications for osteoporosis.
Type of Medium:
Online Resource
ISSN:
0012-1592
,
1440-169X
DOI:
10.1111/dgd.2014.56.issue-9
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2020067-5
SSG:
12
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