In:
PLOS Biology, Public Library of Science (PLoS), Vol. 19, No. 7 ( 2021-7-12), p. e3001333-
Abstract:
SARS-CoV-2 infections are characterized by viral proliferation and clearance phases and can be followed by low-level persistent viral RNA shedding. The dynamics of viral RNA concentration, particularly in the early stages of infection, can inform clinical measures and interventions such as test-based screening. We used prospective longitudinal quantitative reverse transcription PCR testing to measure the viral RNA trajectories for 68 individuals during the resumption of the 2019–2020 National Basketball Association season. For 46 individuals with acute infections, we inferred the peak viral concentration and the duration of the viral proliferation and clearance phases. According to our mathematical model, we found that viral RNA concentrations peaked an average of 3.3 days (95% credible interval [CI] 2.5, 4.2) after first possible detectability at a cycle threshold value of 22.3 (95% CI 20.5, 23.9). The viral clearance phase lasted longer for symptomatic individuals (10.9 days [95% CI 7.9, 14.4] ) than for asymptomatic individuals (7.8 days [95% CI 6.1, 9.7]). A second test within 2 days after an initial positive PCR test substantially improves certainty about a patient’s infection stage. The effective sensitivity of a test intended to identify infectious individuals declines substantially with test turnaround time. These findings indicate that SARS-CoV-2 viral concentrations peak rapidly regardless of symptoms. Sequential tests can help reveal a patient’s progress through infection stages. Frequent, rapid-turnaround testing is needed to effectively screen individuals before they become infectious.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001333
DOI:
10.1371/journal.pbio.3001333.g001
DOI:
10.1371/journal.pbio.3001333.g002
DOI:
10.1371/journal.pbio.3001333.g003
DOI:
10.1371/journal.pbio.3001333.g004
DOI:
10.1371/journal.pbio.3001333.g005
DOI:
10.1371/journal.pbio.3001333.t001
DOI:
10.1371/journal.pbio.3001333.s001
DOI:
10.1371/journal.pbio.3001333.s002
DOI:
10.1371/journal.pbio.3001333.s003
DOI:
10.1371/journal.pbio.3001333.s004
DOI:
10.1371/journal.pbio.3001333.s005
DOI:
10.1371/journal.pbio.3001333.s006
DOI:
10.1371/journal.pbio.3001333.s007
DOI:
10.1371/journal.pbio.3001333.s008
DOI:
10.1371/journal.pbio.3001333.s009
DOI:
10.1371/journal.pbio.3001333.s010
DOI:
10.1371/journal.pbio.3001333.s011
DOI:
10.1371/journal.pbio.3001333.s012
DOI:
10.1371/journal.pbio.3001333.s013
DOI:
10.1371/journal.pbio.3001333.s014
DOI:
10.1371/journal.pbio.3001333.s015
DOI:
10.1371/journal.pbio.3001333.s016
DOI:
10.1371/journal.pbio.3001333.s017
DOI:
10.1371/journal.pbio.3001333.s018
DOI:
10.1371/journal.pbio.3001333.s019
DOI:
10.1371/journal.pbio.3001333.s020
DOI:
10.1371/journal.pbio.3001333.s021
DOI:
10.1371/journal.pbio.3001333.s022
DOI:
10.1371/journal.pbio.3001333.s023
DOI:
10.1371/journal.pbio.3001333.s024
DOI:
10.1371/journal.pbio.3001333.s025
DOI:
10.1371/journal.pbio.3001333.s026
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2126773-X
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