In:
Journal of Fish Diseases, Wiley, Vol. 36, No. 8 ( 2013-08), p. 721-733
Abstract:
Cyprinid herpesvirus 3 ( C y HV ‐3) is the aetiological agent of a serious and notifiable disease afflicting common and koi carp, Cyprinus carpio L., termed koi herpesvirus disease ( KHVD ). Significant progress has been achieved in the last 15 years, since the initial reports surfaced from G ermany, USA and Israel of the C y HV ‐3 virus, in terms of pathology and detection. However, relatively few studies have been carried out in understanding viral replication and propagation. Antibody‐based affinity has been used for detection of C y HV ‐3 in enzyme‐linked immunosorbent assay and PCR ‐based techniques, and immunohistological assays have been used to describe a C y HV ‐3 membrane protein, termed ORF 81. In this study, monoclonal antibodies linked to N ‐hydroxysuccinimide ( NHS )‐activated spin columns were used to purify C y HV ‐3 and host proteins from tissue samples originating in either C y HV ‐3 symptomatic or asymptomatic fish. The samples were next analysed either by polyacrylamide gel electrophoresis ( PAGE ) and subsequently by electrospray ionization coupled to mass spectrometry ( ESI ‐ MS ) or by ESI ‐ MS analysis directly after purification. A total of 78 host proteins and five C y HV ‐3 proteins were identified in the two analyses. These data can be used to develop novel control methods for Cy HV ‐3, based on pathways or proteins identified in this study.
Type of Medium:
Online Resource
ISSN:
0140-7775
,
1365-2761
DOI:
10.1111/jfd.2013.36.issue-8
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
432109-1
detail.hit.zdb_id:
2020444-9
SSG:
21,3
SSG:
12
Permalink