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  • Centre for Evaluation in Education and Science (CEON/CEES)  (2)
  • Gojkovic, Tamara  (2)
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  • Centre for Evaluation in Education and Science (CEON/CEES)  (2)
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  • 1
    Online Resource
    Online Resource
    Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer
    Centre for Evaluation in Education and Science (CEON/CEES) ; 2019
    In:  Journal of Medical Biochemistry Vol. 0, No. 0 ( 2019-10-16)
    Details
    In: Journal of Medical Biochemistry, Centre for Evaluation in Education and Science (CEON/CEES), Vol. 0, No. 0 ( 2019-10-16)
    Abstract: Vitamin D deficiency is repeatedly reported in colorectal cancer (CRC). Since cholesterol and vitamin D share common precursor 7-dehydrocholesterol (7-DHC), it would be important to explore the associations of key vitamin D metabolites and serum lipid parameters in patients with high and low grade CRC. The aim of this study was to analyze relationships between serum 25(OH)D3, 24,25(OH)2D3 and 7-DHC levels and serum lipids in patients with CRC, and to evaluate their potential for prediction of risk for development of high grade CRC. Methods We recruited 82 patients CRC and 77 controls. 7-DHC, 25(OH)D3 and 24,25(OH)2D3 were quantified by LC-MS/MS methods. Results 7-DHC, 25(OH)D3 and vitamin D metabolic ratio (VDMR) were significantly lower in CRC patients than in control group (P 〈 0.001, P 〈 0.010, P 〈 0.050 and P 〈 0.050, respectively). 25(OH)D3 levels were higher in patients with grade I CRC when compared to grade II (P 〈 0.050). All vitamin D metabolites positively correlated with total cholesterol (TC) concentration in CRC patients. 25(OH)D3 was significant predictor of increased CRC risk (P 〈 0.010). After adjustment for TC concentration, 25(OH)D3 lost its predictive abilities. However, 25(OH)D3 remained significant predictor of poorly differentiated type of cancer (P 〈 0.050). Conclusions We found significant positive association between vitamin D status and serum total cholesterol. Although low 25(OH)D3 was found to be a significant risk factor for CRC development, the obtained results primarily suggest profound impact of cholesterol level on vitamin D status in CRC. However, our results suggest that low 25(OH)D3 might independently contribute to development of poorly differentiated tumor.
    Type of Medium: Online Resource
    ISSN: 1452-8266
    URL: Article
    DOI: 10.2478/jomb-2019-0047
    Language: Unknown
    Publisher: Centre for Evaluation in Education and Science (CEON/CEES)
    Publication Date: 2019
    detail.hit.zdb_id: 2405112-3
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Determination of non-cholesterol sterols in serum and HDL fraction by LC/MS-ms: Significance of matrix-related interferences
    Centre for Evaluation in Education and Science (CEON/CEES) ; 2019
    In:  Journal of Medical Biochemistry Vol. 0, No. 0 ( 2019-10-16)
    Details
    In: Journal of Medical Biochemistry, Centre for Evaluation in Education and Science (CEON/CEES), Vol. 0, No. 0 ( 2019-10-16)
    Abstract: Non-cholesterol sterols (NCS) are promising biomarkers for estimation of cholesterol homeostasis properties. In addition, determination of NCS in high-density lipoprotein (HDL) fraction (HDL-NCS) could provide information on cholesterol efflux. However, matrix effects interfere in liquid chromatography–mass spectrometry (LC-MS) analysis of NCS, thereby impairing the method sensitivity. The aims of this study were development, optimization and validation of LC-MS method for quantification of NCS in serum and HDL-NCS. Additionally, matrix effect interferences and methods application in individual serum samples were examined. Methods HDL precipitating reagent was used for HDL isolation. Matrix effect was examined by comparing different surrogates by simple regression analysis. Validation was conducted according to the FDA-ICH guideline. 20 healthy volunteers were recruited for testing of method application. Results The observed matrix effect was 30%, and matrix comparison showed that cholesterol was the dominant contributor to the matrix effect. Cholesterol concentration was adjusted by construction of the calibration curve for serum and HDL fraction (5 mmol/L and 2.5 mmol/L, respectively). The intra- and inter- run variabilities for NCSs were 4.7–10.3% for serum NCS and 3.6–13.6% for HDL-NCS and 4.6–9.5% for serum NCSs and 2.5–9.8% for HDL-NCS, respectively. Recovery studies showed satisfactory results for NCSs: 89.8–113.1% for serum NCS and 85.3–95.8% for HDL-NCS. Conclusions The method was successfully developed and optimized. The matrix interference was solved by customising calibration curves for each method and sample type. The measurement of NCS in HDL fraction was proposed for the first time as potentially useful procedure in biomedical researches.
    Type of Medium: Online Resource
    ISSN: 1452-8266
    URL: Article
    DOI: 10.2478/jomb-2019-0044
    Language: Unknown
    Publisher: Centre for Evaluation in Education and Science (CEON/CEES)
    Publication Date: 2019
    detail.hit.zdb_id: 2405112-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
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