In:
Journal of Cellular and Molecular Medicine, Wiley, Vol. 18, No. 8 ( 2014-08), p. 1580-1587
Abstract:
H irschsprung's disease ( HSCR ), a congenital gastrointestinal disorder, is one of the most common causes of neonatal bowel obstruction. Without an early screening and diagnosis, some patients develop serious complications, such as toxic megacolon or acute enterocolitis. We sought to identify specific serum micro RNA s (mi RNA s) that can serve as novel early, non‐invasive screening signature and then to test their specificity and sensitivity in diagnosing H irschsprung's disease. We obtained serum samples from 95 HSCR cases and 104 matched controls. An initial screening of mi RNA expression was performed through TaqMan Low Density Array. The candidate mi RNA s were validated by individual reverse transcription quantitative real‐time PCR arranged in the training and a two‐stage validation set. Additional double‐blind testing was performed in 23 patients with clinically suspected HSCR to evaluate the diagnostic value and accuracy of the serum mi RNA profile in predicting HSCR . Following a multi‐stage evaluation approach, five mi RNA s were significantly increased in HSCR cases compared with controls. The areas under the receiver operating characteristic ( ROC ) curve of this five‐serum mi RNA signature were 0.895, 0.893 and 0.925 in training set and two validation sets, respectively. The accuracy rate of the five‐mi RNA profile as HSCR signature was 82.6%, which, in the double‐blind testing set, was markedly higher than that of contrast enema (70%), the most commonly used test performed to diagnose HSCR . Our results indicate that a five‐serum mi RNA signature may be linked to HSCR , representing a potential, novel, non‐invasive diagnostic approach for early screening of HSCR .
Type of Medium:
Online Resource
ISSN:
1582-1838
,
1582-4934
DOI:
10.1111/jcmm.2014.18.issue-8
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2076114-4
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