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  • 1
    Online Resource
    Online Resource
    Meiotic failure in male mice lacking an X-linked factor
    Cold Spring Harbor Laboratory ; 2008
    In:  Genes & Development Vol. 22, No. 5 ( 2008-03-01), p. 682-691
    Details
    In: Genes & Development, Cold Spring Harbor Laboratory, Vol. 22, No. 5 ( 2008-03-01), p. 682-691
    Abstract: Meiotic silencing of sex chromosomes may cause their depletion of meiosis-specific genes during evolution. Here, we challenge this hypothesis by reporting the identification of TEX11 as the first X-encoded meiosis-specific factor in mice. TEX11 forms discrete foci on synapsed regions of meiotic chromosomes and appears to be a novel constituent of meiotic nodules involved in recombination. Loss of TEX11 function causes chromosomal asynapsis and reduced crossover formation, leading to elimination of spermatocytes, respectively, at the pachytene and anaphase I stages. Specifically, TEX11-deficient spermatocytes with asynapsed autosomes undergo apoptosis at the pachytene stage, while those with only asynapsed sex chromosomes progress. However, cells that survive the pachytene stage display chromosome nondisjunction at the first meiotic division, resulting in cell death and male infertility. TEX11 interacts with SYCP2, which is an integral component of the synaptonemal complex lateral elements. Thus, TEX11 promotes initiation and/or maintenance of synapsis and formation of crossovers, and may provide a physical link between these two meiotic processes.
    Type of Medium: Online Resource
    ISSN: 0890-9369 , 1549-5477
    URL: Article
    DOI: 10.1101/gad.1613608
    RVK:
    WA 15000
    Language: English
    Publisher: Cold Spring Harbor Laboratory
    Publication Date: 2008
    detail.hit.zdb_id: 806684-X
    detail.hit.zdb_id: 1467414-2
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    A murine replication protein accumulates temporarily in the heterochromatic regions of nuclei prior to initiation of DNA replication
    The Company of Biologists ; 1995
    In:  Journal of Cell Science Vol. 108, No. 3 ( 1995-03-01), p. 927-934
    Details
    In: Journal of Cell Science, The Company of Biologists, Vol. 108, No. 3 ( 1995-03-01), p. 927-934
    Abstract: We have analyzed the expression of the murine P1 gene, the mammalian homologue of the yeast MCM3 protein, during the mitotic cell cycle. The MCM3 protein has previously been shown to be of importance for initiation of DNA replication in Saccharomyces cerevisiae. We found that the murine P1 protein was present in the nuclei of mammalian cells throughout interphase of the cell cycle. This is in contrast to the MCM3 protein, which is located in the nuclei of yeast cells only between the M and the S phase of the cell cycle. Detailed analysis of the intranuclear localization of the P1 protein during the cell cycle revealed that it accumulates transiently in the heterochromatic regions towards the end of G1. The accumulation of the P1 protein in the heterochromatic regions prior to activation of DNA replication suggests that the mammalian P1 protein is also of importance for initiation of DNA replication. The MCM2-3-5 proteins have been suggested to represent yeast equivalents of a hypothetical replication licensing factor initially described in Xenopus. Our data support this model and indicate that the murine P1 protein could function as replication licensing factor. The chromo-somal localization of the P1 gene was determined by fluo-rescence in situ hybridization to region 6p12 in human metaphase chromosomes.
    Type of Medium: Online Resource
    ISSN: 0021-9533 , 1477-9137
    URL: Article
    DOI: 10.1242/jcs.108.3.927
    Language: English
    Publisher: The Company of Biologists
    Publication Date: 1995
    detail.hit.zdb_id: 1410949-9
    detail.hit.zdb_id: 1483099-1
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression
    The Company of Biologists ; 1996
    In:  Journal of Cell Science Vol. 109, No. 1 ( 1996-01-01), p. 143-153
    Details
    In: Journal of Cell Science, The Company of Biologists, Vol. 109, No. 1 ( 1996-01-01), p. 143-153
    Abstract: We have isolated the murine homologue of the human Ki-67 antigen. The Ki-67 antigen is used as a marker to assess the proliferative capacity of tumour cells; however, its cellular function is not known. The murine Ki-67 cDNA sequence (TSG126) was found to contain 13 tandem repeats, making up more than half of the total protein size. A comparison of this repetitive sequence block to its human counterpart, which contains 16 consecutive repeat units, revealed several conserved sequence motifs, including one motif frequently observed in proteins interacting with DNA. An antiserum developed against the product of the TSG126 cDNA clone identified a protein with an apparent molecular mass of 360 kDa, mainly expressed in proliferating cells. The TSG126 protein begins to accumulate during the late G1 stage of the cell cycle and is first seen as numerous small granules evenly distributed throughout the nucleus. During the S and the G2 phases, larger foci that overlap with the nucleoli and the heterochromatic regions are formed. At the onset of mitosis the TSG126 protein undergoes a dramatic redistribution process and becomes associated with the surface of the condensed chromosomes. The relative absence of the TSG126 protein from G1 interphase cells strongly argues against a model where the association of the TSG126 protein with mitotic chromosomes merely reflects a mechanism for the symmetrical distribution of nucleolar proteins between daughter cells. Instead, the intracellular distribution of the TSG126 protein during the cell cycle suggests that it could have a chromatin-associated function in both interphase and mitotic cells. Microinjection of anti-TSG126 antibodies into proliferating Swiss-3T3 fibroblasts was found to delay cell cycle progression, indicating that the TSG126 protein has an essential nuclear function.
    Type of Medium: Online Resource
    ISSN: 0021-9533 , 1477-9137
    URL: Article
    DOI: 10.1242/jcs.109.1.143
    Language: English
    Publisher: The Company of Biologists
    Publication Date: 1996
    detail.hit.zdb_id: 1410949-9
    detail.hit.zdb_id: 1483099-1
    SSG: 12
    Location Call Number Limitation Availability
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