In:
Pathophysiology of Haemostasis and Thrombosis, S. Karger AG, Vol. 23, No. 5 ( 1993), p. 284-292
Abstract:
Platelet dysfunction and increased bleeding tendency has been the most consistently described haemostatic abnormality in patients with renal failure. Besides abnormalities in platelet membrane glycoproteins, a reduced amount of platelet-dense granule content has been demonstrated in patients with end-stage renal failure (ESRD) indicating an acquired storage pool deficiency (SPD) present in uraemia. To study dense granules, platelets were labelled with mepacrine, a fluorescent probe which is specifically incorporated into dense bodies. MepaPlatelets of 13 patients with ESRD and of 11 healthy controls were studied. The results showed that mepacrine-labelled platelets of patients with ESRD reveal a significantly (p 〈 0.05) reduced fluorescence compared to the control group. This implies a reduced number or content of dense granules present in ESRD platelets. Thus, the current data indicate that ESRD is associated with an acquired platelet SPD which may play an important role in the pathophysiology of uraemic bleeding tendency. Moreover, the described FACS assay may be a useful and rapid method for screening patients with suspected acquired or inherited SPD.
Type of Medium:
Online Resource
ISSN:
1424-8832
,
1424-8840
Language:
English
Publisher:
S. Karger AG
Publication Date:
1993
detail.hit.zdb_id:
2081182-2
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