In:
The Journal of Immunology, The American Association of Immunologists, Vol. 198, No. 1_Supplement ( 2017-05-01), p. 131.18-131.18
Abstract:
Sepsis is a serious medical condition caused by an overwhelming immune response to infection and is a major challenge in the intensive care unit, where it’s one of the leading causes of death. Dendritic cells (DCs), acting as sentinels, constantly sense and respond to environmental stimuli. During the progression of sepsis, DCs have been reported to take part in the aberrant immune response and be necessary for survival. Recently, we demonstrated that activation of Aryl Hydrocarbon receptor (AhR), by tryptophan metabolites in DCs, represents a protective mechanism in an experimental animal model of Lipopolysaccharide (LPS)-induced septic shock. Based on these results we examined the role of DCs subsets in sepsis. To determine whether DCs are required for protection against LPS sterile septic shock we used Zbtb46DTR mouse model. We found that Zbtb46DTR chimeras treated with diphtheria toxin are unable to survive after sub-lethal LPS challenge compared to wild-type mice reconstituted with wild-type bone marrow. In addition, naive mice intravenously transferred with LPS-treated DCs survived to a lethal LPS challenge. Notably, we found that mice lacking of CD24+ DCs are extremely susceptible to LPS. Moreover, LPS selectively induces indoleamine-2,3-dioxygenase 1 (IDO1) in bone marrow-derived CD24+. Our data demonstrate for the first time that DCs and specifically CD24+ DCs, play a critical role in sepsis protection.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.198.Supp.131.18
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2017
detail.hit.zdb_id:
1475085-5
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