In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 79, No. 16 ( 2013-08-15), p. 5078-5081
Abstract:
In order to enlarge the substrate binding pocket of the meso -diaminopimelate dehydrogenase from Symbiobacterium thermophilum to accommodate larger 2-keto acids, four amino acid residues (Phe146, Thr171, Arg181, and His227) were targeted for site saturation mutagenesis. Among all mutants, the single mutant H227V had a specific activity of 2.39 ± 0.06 U · mg −1 , which was 35.1-fold enhancement over the wild-type enzyme.
Type of Medium:
Online Resource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/AEM.01049-13
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2013
detail.hit.zdb_id:
223011-2
detail.hit.zdb_id:
1478346-0
SSG:
12
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