In:
Microscopy and Microanalysis, Oxford University Press (OUP), Vol. 6, No. S2 ( 2000-08), p. 858-859
Abstract:
Successful gene therapy is dependent upon well regulated, long-term expression of transgenes in the target tissue. The development and production of very high quality vectors are one of the most important steps leading to delivery of the desired genes. Although several types of vectors have been used for the delivery of transgene to the target cells of the host, our institute routinely uses recombinant viral vectors including adenovirus and adeno-associated virus (rAAV) for gene therapy studies. To assure high quality of the viral vectors being used for animal experiment as well as clinical trials, a number of quality control protocols, including electron microscopy, was developed and implemented to monitor the overall purity, and density of the vectors. Transmission electron microscopy (TEM), combined with optimized procedure of negative staining for virus, offers a unique means to generate visual data of the vector preparation; and it has gained increasing application to help assess the quality of vectors.
Type of Medium:
Online Resource
ISSN:
1431-9276
,
1435-8115
DOI:
10.1017/S1431927600036783
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2000
detail.hit.zdb_id:
1481716-0
SSG:
11
SSG:
12
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