In:
Molecular Nutrition & Food Research, Wiley, Vol. 62, No. 10 ( 2018-05)
Abstract:
After intrinsic labeling of spinach ( Spinacia oleracea L., Chenopodiaceae) with 13 CO 2 , we investigated if labeled polyphenol metabolites were detectable in human plasma. Methods and result In a pilot intervention trial, five healthy men consumed 5 g freeze‐dried 13 C labeled spinach, including a total amount of 160 μmol methoxyflavonols, including 70 μmol 5,3′,4′‐trihydroxy‐3‐methoxy‐6,7‐methylendioxyflavone‐4′‐glucuronide. Plasma samples of all subjects were analyzed with regard to their 13 C/ 12 C ratio. Additionally, 13 C labeled metabolites of patuletin, spinacetin, and 5,3′,4′‐trihydroxy‐3‐methoxy‐6,7‐methylendioxyflavone (TMM) were analyzed in plasma samples in a subgroup of three subjects. TMM‐glucuronide, TMM‐sulfate, and spinacetin‐glucuronide‐sulfate, the latter as 12 C 1 13 C 16 and 13 C 17 isotopologs, were tentatively identified. Plasma concentration of TMM‐glucuronide and TMM‐sulfate reached c max from 19.1–54.3 and 22.5–125.5 nmol L –1 , respectively, 7–9 h post‐ingestion. Conclusion It seems likely that 13 C labeled TMM‐glucuronide and TMM‐sulfate are phase‐II metabolites which were converted after colonic transformation. Variations in plasma kinetics were observed for these two metabolites and may be attributed to the individual composition of the microbiota. We conclude that 13 C labeled polyphenol metabolites are detectable and quantifiable in human plasma.
Type of Medium:
Online Resource
ISSN:
1613-4125
,
1613-4133
DOI:
10.1002/mnfr.201701003
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2160372-8
SSG:
12
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