In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 143, No. Suppl. 1 ( 2007), p. 71-75
Abstract:
〈 i 〉 Background: 〈 /i 〉 IL-9 is an important cytokine in allergic diseases such as asthma, atopic dermatitis, etc. T helper (Th) cells seem to be the main source of IL-9. Cellular and molecular mechanisms of IL-9 production by human Th cells have been poorly understood. 〈 i 〉 Methods: 〈 /i 〉 〈 i 〉 Dermatophagoides farinae 〈 /i 〉 〈 i 〉 (Der f) 〈 /i 〉 -specific Th clones were established from peripheral blood lymphocytes of atopic asthmatics, and cytokine synthesis in response to various stimuli was determined by specific ELISAs. 〈 i 〉 Results: 〈 /i 〉 IL-9 was produced by 14 of 27 human Th clones upon T cell receptor (TCR) stimulation, immobilized anti-CD3 antibody (Ab). IL-9 production was significantly enhanced by the addition of anti-CD28 Ab into the culture, indicating the role of costimulatory signal on IL-9 synthesis. Pharmacologically, IL-9 production was induced by ionomycin (IOM) alone, and enhanced by phorbol 12-myristate 13-acetate (PMA). rIL-2 induced IL-9 production by 8 out of 19 Th clones. IL-9 production by Th clones stimulated with immobilized anti-CD3 Ab was significantly suppressed by the addition of anti-IL-2 neutralizing Ab into the culture. 〈 i 〉 Conclusion: 〈 /i 〉 Approximately half of the 〈 i 〉 Der f 〈 /i 〉 -specific Th clones derived from atopic asthmatics produced IL-9 upon TCR stimulation. Ca 〈 sup 〉 2+ 〈 /sup 〉 signal, CD28 signal, and IL-2 receptor signal seem to play important roles on IL-9 production by human Th cells. Moreover, synthesis of IL-9, a Th2 cytokine, is dependent on IL-2, a Th1 cytokine, which is produced by Th cells themselves.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
2007
detail.hit.zdb_id:
1482722-0
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