In:
Journal of General Virology, Microbiology Society, Vol. 95, No. 8 ( 2014-08-01), p. 1832-1842
Abstract:
Bovine leukemia virus (BLV) induces abnormal B-cell proliferation and B-cell lymphoma in cattle, where the BLV provirus is integrated into the host genome. BLV-infected B-cells rarely express viral proteins in vivo , but short-term cultivation augments BLV expression in some, but not all, BLV-infected B-cells. This observation suggests that two subsets, i.e. BLV-silencing cells and BLV-expressing cells, are present among BLV-infected B-cells, although the mechanisms of viral expression have not been determined. In this study, we examined B-cell markers and viral antigen expression in B-cells from BLV-infected cattle to identify markers that may discriminate BLV-expressing cells from BLV-silencing cells. The proportions of IgM high B-cells were increased in blood lymphocytes from BLV-infected cattle. IgM high B-cells mainly expressed BLV antigens, whereas IgM low B-cells did not, although the provirus load was equivalent in both subsets. Several parameters were investigated in these two subsets to characterize their cellular behaviour. Real-time PCR and microarray analyses detected higher expression levels of some proto-oncogenes (e.g. Maf , Jun and Fos ) in IgM low B-cells than those in IgM high B-cells. Moreover, lymphoma cells obtained from the lymph nodes of 14 BLV-infected cattle contained IgM low or IgM − B-cells but no IgM high B-cells. To our knowledge, this is the first study to demonstrate that IgM high B-cells mainly comprise BLV-expressing cells, whereas IgM low B-cells comprise a high proportion of BLV-silencing B-cells in BLV-infected cattle.
Type of Medium:
Online Resource
ISSN:
0022-1317
,
1465-2099
DOI:
10.1099/vir.0.065011-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
2007065-2
SSG:
12
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