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  • Fuisz, Robert E.  (3)
  • 1965-1969  (3)
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  • 1965-1969  (3)
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  • 1
    Online Resource
    Online Resource
    Kinetic Relations of the Na-Amino Acid Interaction at the Mucosal Border of Intestine
    Rockefeller University Press ; 1967
    In:  The Journal of General Physiology Vol. 50, No. 5 ( 1967-05-01), p. 1261-1286
    Details
    In: The Journal of General Physiology, Rockefeller University Press, Vol. 50, No. 5 ( 1967-05-01), p. 1261-1286
    Abstract: The relation between unidirectional influxes of Na and amino acids across the mucosal border of rabbit ileum was studied under a variety of conditions. At constant Na concentration in the mucosal bathing solution, amino acid influx followed Michaelis-Menten kinetics permitting determination of maximal influx and the apparent Michaelis constant, Kt. Reduction in Na concentration, using choline as substitute cation, caused an increase in Kt for alanine but had no effect on maximal alanine influx. The reciprocal of Kt was a linear function of Na concentration. Similar results were obtained for valine and leucine and these amino acids competitively inhibited alanine influx both in the presence and in the absence of Na. These results lead to a model for the transport system which involves combination of Na and amino acid with a single carrier or site leading to penetration of both solutes. The model predicts that alanine should cause an increase in Na influx and the ratio of this extra Na flux to alanine flux should vary with Na concentration. The observed relation agreed closely with predicted values for Na concentrations from 5 to 140 mM. These results support the hypothesis that interactions between Na and amino acid transport depend in part on a common entry mechanism at the mucosal border of the intestine.
    Type of Medium: Online Resource
    ISSN: 1540-7748 , 0022-1295
    URL: Article
    DOI: 10.1085/jgp.50.5.1261
    Language: English
    Publisher: Rockefeller University Press
    Publication Date: 1967
    detail.hit.zdb_id: 1477246-2
    SSG: 12
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Alanine and Sodium Fluxes Across Mucosal Border of Rabbit Ileum
    Rockefeller University Press ; 1967
    In:  The Journal of General Physiology Vol. 50, No. 5 ( 1967-05-01), p. 1241-1260
    Details
    In: The Journal of General Physiology, Rockefeller University Press, Vol. 50, No. 5 ( 1967-05-01), p. 1241-1260
    Abstract: Unidirectional influxes of L-alanine and Na from the mucosal solution into the epithelium of in vitro rabbit ileum have been determined. In the presence of 140 mM Na, alanine influx is approximately 2.2 µmoles/hr cm2, but is inhibited if the NaCl in the mucosal solution is replaced by choline Cl, Tris-Cl, mannitol, LiCl, or KCl. Although alanine influx is strongly dependent upon Na in the mucosal solution, it is uninfluenced by marked reduction of intracellular Na pools. In addition, alanine influx is unaffected by intracellular alanine concentration. Na influx is markedly inhibited by the presence of Li. Evidence is presented that Na transport across the mucosal border cannot be attributed to simple diffusion even though the net flux across this surface is in the direction of the electrochemical potential difference.
    Type of Medium: Online Resource
    ISSN: 1540-7748 , 0022-1295
    URL: Article
    DOI: 10.1085/jgp.50.5.1241
    Language: English
    Publisher: Rockefeller University Press
    Publication Date: 1967
    detail.hit.zdb_id: 1477246-2
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
  • 3
    Online Resource
    Online Resource
    Amino Acid and Sugar Transport in Rabbit Ileum
    Rockefeller University Press ; 1966
    In:  The Journal of General Physiology Vol. 49, No. 5 ( 1966-05-01), p. 849-866
    Details
    In: The Journal of General Physiology, Rockefeller University Press, Vol. 49, No. 5 ( 1966-05-01), p. 849-866
    Abstract: L-Alanine and 3-O-methyl-D-glucose accumulation by mucosal strips from rabbit ileum has been investigated with particular emphasis on the interaction between Na and these transport processes. L-Alanine is rapidly accumulated by mucosal tissue and intracellular concentrations of approximately 50 mM are reached within 30 min when extracellular L-alanine concentration is 5 mM. Evidence is presented that intracellular alanine exists in an unbound, osmotically active form and that accumulation is an active transport process. In the absence of extracellular Na, the final ratio of intracellular to extracellular L-alanine does not differ significantly from unity and the rate of net uptake is markedly inhibited. Amino acid accumulation is also inhibited by 5 x 10-5 M ouabain. 3-O-methyl-D-glucose accumulation by this preparation is similarly affected by ouabain and by incubation in a Na-free medium. The effects of amino acid accumulation, of ouabain, and of incubation in a Na-free medium on cell water content and intracellular Na and K concentrations have also been investigated. These results are discussed with reference to the two hypotheses which have been suggested to explain the interaction between Na and intestinal nonelectrolyte transport.
    Type of Medium: Online Resource
    ISSN: 1540-7748 , 0022-1295
    URL: Article
    DOI: 10.1085/jgp.49.5.849
    Language: English
    Publisher: Rockefeller University Press
    Publication Date: 1966
    detail.hit.zdb_id: 1477246-2
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
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