In:
Cell Proliferation, Wiley, Vol. 48, No. 4 ( 2015-08), p. 405-412
Abstract:
The aim of this study was to investigate gene expressions of growth factors for angiogenesis, in a three‐dimensional (3D) gel populated with adipose‐derived stromal cells ( ASC s) and endothelial cells ( EC s) in co‐culture. Materials and methods The 3D gel, mixed with green fluorescent protein ( GFP )‐positive ASC s and DsRed‐Express‐positive EC s, 1:1 ratio, was established in vitro. The phenomenon of angiogenesis was observed using confocal microscopy. To detect gene expressions for growth factor proteins in both ASC s and EC s, transwell co‐culture was used, and cell lysate samples were collected at 1, 3, 5 and 7 days. Semi‐quantitative polymerase chain reaction ( PCR ) was conducted to quantify mRNA expressions of the growth factors. Results Angiogenesis was first observed in the gels by 7 days post‐implantation. Over this time in EC s, genes coding for VEGFA /B, IGF ‐1, HIF ‐1α, FGF ‐1/‐2 and BMP ‐5/‐7 significantly increased. Meanwhile in ASC s, genes coding for VEGFA /B, IGF ‐1, HIF ‐1α, FGF ‐1/‐2 and BMP ‐6 also were significantly enhanced. In particular, increased amounts of IGF ‐1 and HIF ‐1α in both EC s and ASC s were prominent relative to other factors. Conclusions Contact co‐culture with ASC s and EC s at 1:1 ratio, in the 3D gel promoted angiogenesis; non‐contact co‐culture further confirmed gene expressions for growth factors, VEGFA / B , IGF ‐1 , HIF ‐1α and FGF ‐1/‐2 in both ASC s and EC s; BMP ‐5/‐7 in EC s and BMP ‐6 in ASC s were also confirmed. This establishment of growth factor expression seemed to be responsible for enhancement of angiogenesis. This indicates that these factors could be utilized as targets for engineered angiogenesis.
Type of Medium:
Online Resource
ISSN:
0960-7722
,
1365-2184
DOI:
10.1111/cpr.2015.48.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2019986-7
SSG:
12
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