In:
BioMed Research International, Hindawi Limited, Vol. 2020 ( 2020-10-08), p. 1-7
Kurzfassung:
Introduction . The rate of thawing of cryopreserved human iliac arteries allografts (CHIAA) directly affects the severeness of structural changes that occur during this process. Method . The experiment was performed on ten CHIAA. The 10% dimethylsulphoxide in 6% hydroxyethyl starch solution was used as the cryoprotectant; all CHIAA were cooled at a controlled rate and stored in the vapor phase of liquid nitrogen (-194°C). Two thawing protocols were tested: (1) placing the CHIAA in a water bath at 37°C, and (2) the CHIAA were thawed in a controlled environment at 5°C. All samples underwent analysis under a scanning electron microscope. Testing of the mechanical properties of the CHIAA was evaluated on a custom-built single axis strain testing machine. Longitudinal and circumferential samples were prepared from each tested CHIAA. Results . Ultrastructural analysis revealed that all five CHIAA thawed during the thawing protocol 1 which showed significantly more damage to the subendothelial structures when compared to the samples thawed in protocol 2. Mechanical properties: Thawing protocol 1—longitudinal UTS 2 , 53 ± 0 , 47 MPa at relative strain 1 , 27 ± 0 , 12 and circumferential UTS 1 , 94 ± 0 , 27 MPa at relative strain 1 , 33 ± 0 , 09 . Thawing protocol 2—longitudinal ultimate tensile strain (UTS) 2 , 42 ± 0 , 34 MPa at relative strain 1 , 32 ± 0 , 09 and circumferential UTS 1 , 98 ± 0 , 26 MPa at relative strain 1 , 29 ± 0 , 07 . Comparing UTS showed no statistical difference between thawing methods. Conclusion . Despite the significant differences in structural changes of presented thawing protocols, the ultimate tensile strain showed no statistical difference between thawing methods.
Materialart:
Online-Ressource
ISSN:
2314-6133
,
2314-6141
DOI:
10.1155/2020/6545190
Sprache:
Englisch
Verlag:
Hindawi Limited
Publikationsdatum:
2020
ZDB Id:
2698540-8
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