In:
Genome Research, Cold Spring Harbor Laboratory, Vol. 15, No. 2 ( 2005-02), p. 302-311
Abstract:
We used arrays of 2069 BACs (1303 nonredundant autosomal clones) to map sequence variation among Mus spretus (SPRET/Ei and SPRET/Glasgow) and Mus musculus (C3H/HeJ, BALB/cJ, 129/J, DBA/2J, NIH, FVB/N, and C57BL/6) strains. We identified 80 clones representing 74 autosomal loci of copy number variation (|log 2 ratio| ≥ 0.4). These variant loci distinguish laboratory strains. By FISH mapping, we determined that 63 BACs mapped to a single site on C57BL/6J chromosomes, while 17 clones mapped to multiple chromosomes ( n = 16) or multiple sites on one chromosome ( n = 1). We also show that small ratio changes (Δ log 2 ratio ∼ 0.1) distinguish homozygous and heterozygous regions of the genome in interspecific backcross mice, providing an efficient method for genotyping progeny of backcrosses.
Type of Medium:
Online Resource
ISSN:
1088-9051
Language:
English
Publisher:
Cold Spring Harbor Laboratory
Publication Date:
2005
detail.hit.zdb_id:
1483456-X
SSG:
12
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