In:
European Journal of Oral Sciences, Wiley, Vol. 114, No. 2 ( 2006-04), p. 147-153
Abstract:
In the present study, a proteomic approach was applied to evaluate the influence of salivary protein composition on in vitro dental pellicle formation and its possible correlation with dental caries. Whole saliva, collected from caries‐free and caries‐susceptible subjects, was analyzed by two‐dimensional electrophoresis, and protein spots were identified by mass spectrometry. Data analysis of salivary protein composition showed a statistically significant correlation between the quantity of acidic proline‐rich proteins (PRPs), lipocalin, cystatin SN and cystatin S, and samples from the caries‐free group of subjects [decayed, missing or filled teeth (DMFT) = 0]. Samples from subjects with a high DMFT index appear to be correlated with high levels of amylase, immunoglobulin A, and lactoferrin. In vitro pellicle‐composition experiments showed the same correlations found for whole saliva. As cystatins are known physiological inhibitors of cathepsins, the higher quantities of lipocalin, and cystatins S and SN found in the samples from the caries‐free subjects suggest that inhibition of proteolytic events on other salivary proteins may indirectly provide tooth protection. The correlation between higher levels of the phosphorylated acidic PRPs 1/2 with samples from the caries‐free group also suggests a protective role for these proteins.
Type of Medium:
Online Resource
ISSN:
0909-8836
,
1600-0722
DOI:
10.1111/eos.2006.114.issue-2
DOI:
10.1111/j.1600-0722.2006.00328.x
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
2025657-7
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