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    Online Resource
    Efeito do transporte no desenvolvimento de embriões bovinos cultivados in vitro a fresco ou reaquecidos após vitrificação
    FapUNIFESP (SciELO) ; 2006
    In:  Revista Brasileira de Zootecnia Vol. 35, No. 6 ( 2006-12), p. 2285-2289
    Details
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 35, No. 6 ( 2006-12), p. 2285-2289
    Abstract: The aim of this study was to evaluate the viability of in vitro produced bovine embryos, fresh or warmed, after submitted to different periods of transportation (6h-12h). Oocytes obtained from ovaries collected from slaughterhouse were matured, fertilized and cultured in vitro. After seven days, grades I and II blastocysts (according to IETS manual) were selected and vitrified after exposition to PBS solution with 5% fetal calf serum (HM), added with 10% ethylene glycol (EG) and 10% of dymetil sulfoxide (DMSO), for one minute, followed by HM solution with 20% EG and 20% DMSO, for 20 seconds. Embryos were loaded into open pulled straws (OPS) and plunged into liquid nitrogen. Warming was performed at 39ºC by embryo exposure to decreasing concentration of sucrose (0.25 and 0.15M), for five minutes in each step. The warmed embryos were distributed in three groups: V0: in vitro cultured after warmed; V6: embryos loaded into straws and kept for 6 hours at 35ºC, before in vitro culture; and V12: embryos loaded into straws and kept for 12 hours at 35ºC, before in vitro culture. Each group was evaluated by control groups of fresh embryos (C0, C6 and C12, respectively). The embryos were co-cultured with cumulus cells in TCM-199 micro droplets added with SFB. Re-expansion and hatching rates after 48 hours in culture were evaluated and results were compared by the Chi-square test. Re-expanded rates among groups V0, V6 and V12 as well as hatching rates among vitrified groups and among control groups did not differ. However, hatching rates were different between vitrified groups and their respective controls. The satisfactory rates of hatching suggest that it is possible to transport warmed and fresh in vitro produced embryos for periods up to 12 hours.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    URL: Article
    DOI: 10.1590/S1516-35982006000800012
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2006
    detail.hit.zdb_id: 2078814-9
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  • 2
    Online Resource
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    Efeito da somatotropina na população folicular, recuperação de oócitos e produção in vitro de embriões em vacas Gir
    FapUNIFESP (SciELO) ; 2007
    In:  Revista Brasileira de Zootecnia Vol. 36, No. 2 ( 2007-04), p. 380-386
    Details
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 36, No. 2 ( 2007-04), p. 380-386
    Abstract: The aim of this study was to evaluate ovarian pre-stimulation using recombinant bovine somatotropin (rbST) or rbST associated to FSH on follicular population, oocyte retrieval and in vitro embryo production in Gir cows (Bos indicus). Estrous cycle of non-lactating cycling cows with similar body and reproductive conditions were synchronized with cloprostenol followed by norgestomet ear implants, replaced every fourteen days. The animals were randomly distributed in three treatments: TI or control (punctured without pre-stimulation), TII (injection of 160 mg of rbST, before each aspiration) and TIII (injection of 160 mg of rbST followed by administration of 250 U.I. of FSH, before each aspiration). Oocytes were maturated and fertilized in vitro and presumptive zygotes were cultured in vitro for 192 hours post-fertilization. Treatments did not differ for ovarian follicular population. However, an increase in the diameter of the largest follicle and in the number of large and medium follicles with reduction in the number of small follicles was observed for TIII. Oocyte recovery did not differ between TI and TIII, but recovery rate was lower for TII. The number of grade I oocytes was higher and the number of degenerated oocytes was lower for TIII than for TI or TII. The rbST increased cleavage rate and blastocyst production, and when associated to FSH the quality of oocyte in Gir animals was improved.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    URL: Article
    DOI: 10.1590/S1516-35982007000200015
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2007
    detail.hit.zdb_id: 2078814-9
    Location Call Number Limitation Availability
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