In:
Journal of Histochemistry & Cytochemistry, SAGE Publications, Vol. 12, No. 4 ( 1964-04), p. 293-297
Abstract:
The preparation of a new substrate for β-glucuronidase, naphthol AS-BI β-d-glucuronide, has been described. The potassium salt of naphthol AS-BI and acetobromomethyl glucuronate were reacted in ethanol. Unreacted anilide was removed and deacetylation and deesterification were carried out with barium hydroxide. The barium salt of naphthol AS-BI glucuronide was separated and was then converted to free acid. The compound has an elemental analysis which agrees with the theoretical one. It has also been possible to devise a satisfactory cytochemical technique for the in situ demonstration of β-glucuronidase employing sodium salt of naphthol AS-BI glucuronide and hexazonium pararosanilin. The optimal staining reaction was obtained with 0.25 mM substrate and 1.8 mM diazo reagent at pH 5.2 in 20 to 30 minutes at 37°C for rat liver and kidney. The brilliant red dye was visualized at the site of enzyme activity mostly as discrete granules. A brief discussion concerned the evaluation of the present method as a cytochemical technique for the demonstration of β-glucuronidase.
Type of Medium:
Online Resource
ISSN:
0022-1554
,
1551-5044
Language:
English
Publisher:
SAGE Publications
Publication Date:
1964
detail.hit.zdb_id:
1421306-0
SSG:
12
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