In:
Infectious Disorders - Drug Targets, Bentham Science Publishers Ltd., Vol. 19, No. 1 ( 2019-02-04), p. 81-87
Abstract:
The research was conducted to study 1188 AC polymorphism of Interleukin
(IL)-12B gene for C/C, A/C and A/A genotypes in families of Hepatitis C virus (HCV) infected patients in Egypt. Methods: Three hundreds HCV patients, 860 family members and 100 healthy subjects
were studied. All family members were screened for HCV antibodies by enzyme-linked immunosorbent assay (ELISA). Positive cases were examined using Real-Time polymerase
chain reaction (PCR) to confirm the presence of HCV ribonucleic acid (RNA) and detect the viral load. Molecular study of IL-12B gene was carried out on all patients, family
members and controls using PCR and restriction enzyme analysis. Results: HCV infection was confirmed in 10.6% of family members. The distribution of
IL-12B gene polymorphism in patients was 2.3%, 45.7% and 52% for C/C, A/C and A/A genotypes respectively, in infected family members was 3.3%, 41.7%, 55%, in noninfected
family members was 4.5%, 43.5% and 52% for C/C, A/C and A/A genotypes respectively and in control was 5%, 36% and 59% for C/C, A/C and A/A genotypes respectively.
The frequency of the C/C, A/C and A/A genotype was not significantly different between the studied groups. Conclusion: IL-12B gene polymorphism has no role in intrafamilial susceptibility of
HCV transmission. The distribution of the functional 1188 AC polymorphism of Interleukin (IL)-12B gene for C/C, A/C and A/A genotypes was not significantly different
among the studied groups.
Type of Medium:
Online Resource
ISSN:
1871-5265
DOI:
10.2174/1871526518666171227210541
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2019
detail.hit.zdb_id:
2234298-9
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