In:
Microbial Biotechnology, Wiley, Vol. 10, No. 6 ( 2017-11), p. 1365-1375
Abstract:
Recently, syngas has gained significant interest as renewable and sustainable feedstock, in particular for the biotechnological production of poly([ R ]‐3‐hydroxybutyrate) ( PHB ). PHB is a biodegradable, biocompatible polyester produced by some bacteria growing on the principal component of syngas, CO . However, working with syngas is challenging because of the CO toxicity and the explosion danger of H 2 , another main component of syngas. In addition, the bioprocess control needs specific monitoring tools and analytical methods that differ from standard fermentations. Here, we present a syngas fermentation platform with a focus on safety installations and process analytical technology ( PAT ) that serves as a basis to assess the physiology of the PHB ‐producing bacterium Rhodospirillum rubrum . The platform includes (i) off‐gas analysis with an online quadrupole mass spectrometer to measure CO consumption and production rates of H 2 and CO 2 , (ii) an at‐line flow cytometer to determine the total cell count and the intracellular PHB content and (iii) different online sensors, notably a redox sensor that is important to confirm that the culture conditions are suitable for the CO metabolization of R. rubrum . Furthermore, we present as first applications of the platform a fed‐batch and a chemostat process with R. rubrum for PHB production from syngas.
Type of Medium:
Online Resource
ISSN:
1751-7915
,
1751-7915
DOI:
10.1111/mbt2.2017.10.issue-6
DOI:
10.1111/1751-7915.12727
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2406063-X
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