In:
American Journal of Veterinary Research, American Veterinary Medical Association (AVMA), Vol. 63, No. 8 ( 2002-08-01), p. 1134-1139
Abstract:
Objective —To determine the effects of recombinant equine interleukin -1β (reIL-1β) and 4 anti-inflammatory compounds on the expression and activity of cyclooxygenase (COX)-2 in cultured equine chondrocytes. Sample Population —Articular cartilage from 9 young adult horses. Procedure —Reverse transcriptase-polymerase chain reaction methods were used to amplify a portion of equine COX-2 to prepare a cDNA probe. Northern blot analysis was used to quantify the expression of COX-2 in first-passage cultures of equine articular chondrocytes propagated in media containing dexamethasone (DEX), phenylbutazone (PBZ), polysulfated glycosaminoglycan, and hyaluronan, each at concentrations of 10 and 100 µg/ml and each with or without reIL-1β. A commercial immunoassay was used to determine prostaglandin E 2 (PGE 2 ) concentrations in conditioned medium of similarly treated cells to quantify COX-2 activity. Results —Addition of reIL-1β increased the expression of COX-2 in a dose-dependent manner, which was paralleled by an increased concentration of PGE 2 in culture medium. Concentration of PGE 2 in spent medium from reIL-1β-treated chondrocytes was significantly reduced by DEX and PBZ; however, only DEX significantly reduced gene expression of COX-2. Conclusions and Clinical Relevance —Prostaglandin E 2 is considered to be an important mediator in the pathophysiologic processes of arthritis, and cultured chondrocytes respond to interleukin-1 with enhanced expression and activity of COX-2. Palliative relief in affected horses is probably attributable, in part, to inhibition of PGE 2 synthesis; however, analysis of these data suggests that of the 4 compounds tested, only DEX affects pretranslational regulation of the COX-2 gene in cultured equine chondrocytes. ( Am J Vet Res 2002;63:1134–1139)
Type of Medium:
Online Resource
ISSN:
0002-9645
DOI:
10.2460/ajvr.2002.63.1134
Language:
Unknown
Publisher:
American Veterinary Medical Association (AVMA)
Publication Date:
2002
detail.hit.zdb_id:
2056942-7
SSG:
22
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