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  • Du, Wenwen  (4)
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  • 1
    Online Resource
    Online Resource
    Comprehensive Identification of Reliable Reference Genes for qRT-PCR Normalization of Fusarium oxysporum-Resistant Genes’ Expressions in Lilium sargentiae Wilson
    MDPI AG ; 2023
    In:  Applied Sciences Vol. 13, No. 6 ( 2023-03-07), p. 3395-
    Details
    In: Applied Sciences, MDPI AG, Vol. 13, No. 6 ( 2023-03-07), p. 3395-
    Abstract: Fusarium wilt (caused by Fusarium oxysporum f. sp. Lilii) is one of the most damaging diseases in lily (Lilium sargentiae Wilson). Although some F. oxysporum-resistant lily varieties have been identified and are being utilized in resistant breeding, the regulation network of the resistance-associated mechanisms is yet to be studied due to the lack of reliable reference genes for qRT-PCR (quantitative reverse transcription PCR) normalization. The reliability of results by qRT-PCR relies mainly on the stability of the reference genes. This study investigated the reliability of nine candidate reference genes (CYP, EF1-α, GAPDH, TUB, UBQ, AQP, HIS, PGK, and RPL13) for qRT-PCR analysis of F. oxysporum-resistant genes. Expression stability analysis via common programs GeNorm, BestKeeper, and NormFinder, at different time points post-inoculation of F. oxysporum, revealed that all nine genes met the basic requirements of reference genes. Amongst them, HIS and GAPDH displayed the highest and the lowest expression stability, respectively. The reliability of HIS was further validated by analyzing the expression levels of four resistance-related candidate genes. The expression patterns of the four target genes were consistent with their responses to pathogenetic fungi in other plants. Our results show that HIS is the most suitable reference gene for accurately normalizing F. oxysporum-resistant genes’ expressions in L. sargentiae.
    Type of Medium: Online Resource
    ISSN: 2076-3417
    URL: Article
    DOI: 10.3390/app13063395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2704225-X
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  • 2
    Online Resource
    Online Resource
    Transcriptome Analysis of Key Genes Involved in Color Variation between Blue and White Flowers of Iris bulleyana
    Hindawi Limited ; 2023
    In:  BioMed Research International Vol. 2023 ( 2023-1-18), p. 1-13
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2023 ( 2023-1-18), p. 1-13
    Abstract: Iris bulleyana Dykes (Southwest iris) is an extensively distributed Iridaceae species with blue or white flowers. Hereby, we performed a systematic study, employing metabolomics and transcriptomics to uncover the subtle color differentiation from blue to white in Southwest iris. Fresh flower buds from both cultivars were subjected to flavonoid/anthocyanin and carotenoid-targeted metabolomics along with transcriptomic sequencing. Among 297 flavonoids, 24 anthocyanins were identified, and 13 showed a strong down-accumulation pattern in the white flowers compared to the blue flowers. Significant downregulation of 3GT and 5GT genes involved in the glycosylation of anthocyanins was predicted to hinder the accumulation of anthocyanins, resulting in white coloration. Besides, no significant altered accumulation of carotenoids and expression of their biosynthetic genes was observed between the two cultivars. Our study systematically addressed the color differentiation in I. bulleyana flowers, which can aid future breeding programs.
    Type of Medium: Online Resource
    ISSN: 2314-6141 , 2314-6133
    URL: Article
    DOI: 10.1155/2023/7407772
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2698540-8
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  • 3
    Online Resource
    Online Resource
    Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco
    MDPI AG ; 2023
    In:  Genes Vol. 14, No. 10 ( 2023-10-09), p. 1920-
    Details
    In: Genes, MDPI AG, Vol. 14, No. 10 ( 2023-10-09), p. 1920-
    Abstract: Flavonoid-3′,5′-hydroxylase (F3′5′H) is the key enzyme for the biosynthesis of delphinidin-based anthocyanins, which are generally required for purple or blue flowers. Previously, we isolated a full-length cDNA of PgF3′5′H from Platycodon grandiflorus, which shared the highest homology with Campanula medium F3′5′H. In this study, PgF3′5′H was subcloned into a plant over-expression vector and transformed into tobacco via Agrobacterium tumefaciens to investigate its catalytic function. Positive transgenic tobacco T0 plants were obtained by hygromycin resistance screening and PCR detection. PgF3′5′H showed a higher expression level in all PgF3′5′H transgenic tobacco plants than in control plants. Under the drive of the cauliflower mosaic virus (CaMV) 35S promoter, the over-expressed PgF3′5′H produced dihydromyricetin (DHM) and some new anthocyanin pigments (including delphinidin, petunidin, peonidin, and malvidin derivatives), and increased dihydrokaempferol (DHK), taxifolin, tridactyl, cyanidin derivatives, and pelargonidin derivatives in PgF3′5′H transgenic tobacco plants by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis, resulting in a dramatic color alteration from light pink to magenta. These results indicate that PgF3′5′H products have F3′5′H enzyme activity. In addition, PgF3′5′H transfer alters flavonoid pigment synthesis and accumulation in tobacco. Thus, PgF3′5′H may be considered a candidate gene for gene engineering to enhance anthocyanin accumulation and the molecular breeding project for blue flowers.
    Type of Medium: Online Resource
    ISSN: 2073-4425
    URL: Article
    DOI: 10.3390/genes14101920
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2527218-4
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  • 4
    Online Resource
    Online Resource
    Application of Morphological and Physiological Markers for Study of Drought Tolerance in Lilium Varieties
    MDPI AG ; 2022
    In:  Horticulturae Vol. 8, No. 9 ( 2022-08-30), p. 786-
    Details
    In: Horticulturae, MDPI AG, Vol. 8, No. 9 ( 2022-08-30), p. 786-
    Abstract: The shortage of water resources is an unfavourable factor that restricts the production of flowers. The use of drought-resistant morphological markers is of great significance to distinguish the drought resistance of flower varieties. In this paper, we study the difference in drought tolerance of seven common lily varieties in the flower market by morphological and physiological markers. The results showed that there were differences in leaf morphological indices and anatomical structures among the seven varieties. Drought reduced the chlorophyll content, inhibited the photosynthetic rate, and increased catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), malondialdehyde (MDA), proline, soluble sugar, and soluble protein. After rewatering, the activities of CAT, POD, and SOD of ‘Lyon’, ‘Royal Sunset’, and ‘Robina’ varieties decreased, which was opposite to the varieties of ‘Immaculate’, ‘Elena’, ‘Siberia’, and ‘Gelria’. According to the membership function value of physiological indices, the drought resistance of seven lily varieties from weak to strong was ‘Immaculate’, ‘Elena’, ‘Siberia’, ‘Gelria’, ‘Robina’, ‘Royal Sunset’, and ‘Lyon’. Drought resistance is related to the thickness of leaves, palisade tissue, sponge tissue, and specific leaf area. Lily leaf structure can be used as one of the indices to judge drought resistance.
    Type of Medium: Online Resource
    ISSN: 2311-7524
    URL: Article
    DOI: 10.3390/horticulturae8090786
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2813983-5
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