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  • 1
    In: Agricultural and Forest Entomology, Wiley, Vol. 22, No. 1 ( 2020-02), p. 41-49
    Abstract: Migratory insect pests pose a substantial challenge to global food security. These issues are particularly acute when pest incursions occur considerably beyond the expected range, through natural migration or human‐aided transport, because the lack of species‐specific control strategies and a potential absence of species‐specific natural enemies in the newly‐invaded area may lead to rapid establishment of a new pest. One such threat is posed by the Oriental migratory locust Locusta migratoria manilensis in China, which, historically, has been restricted to eastern China from the Bohai Gulf southwards, and now threatens to expand its range into the agriculturally important region of northeast China. We analyzed data from a recent outbreak of migratory locusts in Heilongjiang Province (extreme northeast China), 〉  700 km north of its current known range, and identified the source region, timing of arrival and probable migratory routes of this incursion. We further show that warming temperatures in this region will likely allow subsequent invasions to establish permanent populations in northeast China, and thus authorities in this important crop‐producing region of East Asia should be vigilant to the threat posed by this species.
    Type of Medium: Online Resource
    ISSN: 1461-9555 , 1461-9563
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2016391-5
    SSG: 23
    SSG: 12
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  • 2
    In: Insects, MDPI AG, Vol. 10, No. 6 ( 2019-05-30), p. 154-
    Abstract: The spotted alfalfa aphid (Therioaphis trifolii (Monell)) is a known destructive pest that can significantly reduce alfalfa yields. Two differentially up-regulated alfalfa trypsin inhibitors ‘Msti-94’ and ‘Msti-16’ in transcriptome were verified in terms of their mRNA levels using RT-qPCR. The prokaryotic expression vector was constructed and its biological functions, including phenotypic and physiological responses, were verified through feeding spotted alfalfa aphids with active recombinant protein mixed with an artificial diet. Gene clone and gene prokaryotic expression confirmed that Msti-94 had a size of 651 bp, encoded 216 amino acids with a predicted protein weight of 23.5 kDa, and a pI value of 6.91. Similarly, the size of Msti-16 was 612 bp, encoded 203 amino acids, and had a predicted protein weight of 22.2 kDa with a pI value of 9.06. We concluded that both Msti-94 and Msti-16 acted as a stomach poison with survival rates reduced to 21.7% and 18.3%, respectively, as compared to the control, where the survival rate was significantly (p 〈 0.05) higher (60.0%). Aphid reproduction rates were significantly reduced, after 72 h of feeding, in both the Msti-94 and Msti-16 treatments compared to the controls. A concentration of 800 μg/mL (0.8 mg/mL) of recombinant protein and 5000 μg/mL (5 mg/mL) of recombinant expressing bacteria that inhibits the total protease, which ultimately disrupted the activity of trypsin, chymotrypsin, and aminopeptidase.
    Type of Medium: Online Resource
    ISSN: 2075-4450
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2662247-6
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  • 3
    In: Insects, MDPI AG, Vol. 13, No. 1 ( 2022-01-10), p. 72-
    Abstract: Oedaleus decorus asiaticus (Bey-Bienko) is a destructive pest in grasslands and adjacent farmland in northern China, Mongolia, and other countries in Asia. It has been supposed that this insect pest can migrate a long distance and then induce huge damages, however, the migration mechanism is still unrevealed. The current study uses insect light trap data from Yanqing (Beijing), together with regional meteorological data to determine how air flow contributes to the long-distance migration of O. decorus asiaticus. Our results indicate that sinking airflow is the main factor leading to the insects’ forced landing, and the prevailing northwest wind was associated with O. decorus asiaticus taking off in the northwest and moving southward with the airflow from July to September. Meanwhile, the insects have a strong migratory ability, flying along the airflow for several nights. Thus, when the airflow from the northwest met the northward-moving warm current from the southwest, a large number of insects were dropped due to sinking airflow, resulting in a large outbreak. Our simulations suggest that the source of the grasshoppers involved in these outbreaks during early 2000s in northern China probably is in Mongolia, and all evidence indicates that there are two important immigrant routes for O. decorus asiaticus migration from Mongolia to Beijing. These findings improves our understanding of the factors guiding O. decorus asiaticus migration, providing valuable information to reduce outbreaks in China that have origins from outside the country.
    Type of Medium: Online Resource
    ISSN: 2075-4450
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662247-6
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  • 4
    In: Frontiers in Physiology, Frontiers Media SA, Vol. 12 ( 2021-3-22)
    Abstract: In order to explore the synergistic control effect of crude extracts of Artemisia sieversiana and Metarhizium anisopliae on Oedaleus asiaticus , we used different doses of M. anisopliae and crude extracts of A. sieversiana singly and in combination, to determine their toxicities to fourth instar O. asiaticus . The results showed that the combination of 10% crude extract of A. sieversiana with 10 7 and 10 8 spores/g M. anisopliae concentrations and the combination of 20% crude extract of A. sieversiana with 10 7 and 10 8 spores/g M. anisopliae concentrations had significant effects on the mortality, body weight gain, body length gain, growth rate, and overall performance of O. asiaticus than those of the crude extract of A. sieversiana and M. anisopliae alone. Among them, the 20% A. sieversiana crude extract mixed with 10 8 spores/g M. anisopliae and 10% A. sieversiana crude extract combined with 10 7 spores/g M. anisopliae , had the best control efficacy. In order to clarify the biochemical mechanism underlying the immune responses of O. asiaticus to the pesticide treatments, we monitored the activities of four enzymes: superoxidase dismutase (SOD), peroxidase (POD), catalase (CAT), and polyphenol oxidase (PPO). The results showed that the activities of three enzymes (SOD, CAT, and PPO) were significantly increased from the treatment with the combination of M. anisopliae mixed with crude extract of A. sieversiana . Interestingly, compared to the crude extract, the combination treatment did not significantly induce the expression of POD enzyme activity, which may be a biochemical factor for increasing the control effect of the combination treatment. Our results showed that the combination treatment had synergistic and antagonistic effects on host mortality, growth, development, and enzyme activities in O. asiaticus .
    Type of Medium: Online Resource
    ISSN: 1664-042X
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2564217-0
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