In:
FEBS Letters, Wiley, Vol. 462, No. 1-2 ( 1999-11-26), p. 61-65
Abstract:
Fusion proteins between the human A 1 adenosine receptor and the pertussis toxin resistant (Cys351Gly) mutant of the G‐protein α subunit G i1 α (A1/Gi), and between the human A 1 adenosine receptor, the Aequorea victoria green fluorescent protein (GFP) and Cys351Gly G i1 α (A1/GFP/Gi), were expressed in CHO cells. The agonist NECA caused a stimulation of [ 35 S]GTPγS binding at both fusion proteins with similar concentration dependence as at the native receptor. However in the presence of pertussis toxin NECA stimulation of [ 35 S]GTPγS binding was only seen at the A1/GFP/Gi fusion protein. The regulation of the adenylyl cyclase and MAP kinase effector systems by both fusion proteins was attenuated following pertussis toxin treatment. These studies demonstrate for the first time the characterisation of a fusion protein between a G‐protein coupled receptor, GFP and a G‐protein α subunit.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(99)01467-2
Language:
English
Publisher:
Wiley
Publication Date:
1999
detail.hit.zdb_id:
1460391-3
SSG:
12
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