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  • Doita, Minoru  (2)
  • 2005-2009  (2)
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  • 2005-2009  (2)
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  • 1
    In: Journal of Orthopaedic Research, Wiley, Vol. 24, No. 6 ( 2006-06), p. 1271-1278
    Abstract: To investigate the efficacies and the longevity of RNA interference in nucleus pulposus cells from rat and human, two reporter luciferase plasmids ( Firefly and Renilla ) were used. These plasmids were cotransfected with siRNA targeting Firefly luciferase to the nucleus pulposus cells extracted from Sprague Dawley rats and scoliosis patients. The inhibitory effects were evaluated by dual luciferase assay for 3 weeks. Proliferation activity of fibroblast‐like cells extracted from the subcutaneous tissue of Sprague Dawley rats and the nucleus pulposus cells were measured by proliferation assay (WST‐8 assay) every 2 days after plating. The expression of Firefly luciferase was drastically inhibited both in rats (94.7%) and in humans (93.7%). The inhibitory effects were maintained for 2 weeks and had disappeared completely by 3 weeks. The proliferation activity of nucleus pulposus cells was significantly lower than fibroblast‐like cells. We have shown, for the first time, siRNA‐mediated gene silencing in rat and human disc cells for a relatively sustained period, probably due to the stability of the nucleus pulposus cells in terms of cell proliferation. The demonstration of this study may allow further exploration of the use of siRNA for scientific research and the treatment of disc degenerative diseases. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 24:1271–1278, 2006
    Type of Medium: Online Resource
    ISSN: 0736-0266 , 1554-527X
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2006
    detail.hit.zdb_id: 2050452-4
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  • 2
    In: Journal of Neurosurgery: Spine, Journal of Neurosurgery Publishing Group (JNSPG), Vol. 8, No. 2 ( 2008-02), p. 186-192
    Abstract: ✓ The suggested methods of treatment for spondyloptosis have included benign neglect, in situ fusion and variations, decompression and fusion, and vertebrectomy (the Gaines procedure). On review of the literature, the authors found no previous report in the English-language literature in which external fixation was used in the treatment of spondyloptosis. This 33-year-old woman with spondyloptosis underwent a 2-stage operation involving decompression, reduction, and posterior fusion in which an Ilizarov external fixator and transpedicular fixation system were used. Spondylolisthesis with a slippage of angle 78° and 〉 100% slippage was partially reduced to 30° and 60% without neurological alterations and without complications. The postoperative follow-up showed marked improvement in her symptoms and a good cosmetic result. Reconstructed computed tomography scanning at 18 months demonstrated complete fusion. The use of external fixation in the treatment of spondyloptosis may be preferable because of its neurological safety, despite the longer duration of treatment, than single-stage operation. The authors believe posterior decompression of the cauda equina, partial reduction of the spondylolisthetic deformity, interbody fusion, and stabilization with an external fixator and transpedicular fixation system can be successfully and safely used as a 2-stage treatment for adult high-grade spondyloptosis.
    Type of Medium: Online Resource
    ISSN: 1547-5654
    RVK:
    Language: Unknown
    Publisher: Journal of Neurosurgery Publishing Group (JNSPG)
    Publication Date: 2008
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