In:
eLife, eLife Sciences Publications, Ltd, Vol. 5 ( 2016-11-25)
Abstract:
Axolotls are unique in their ability to regenerate the spinal cord. However, the mechanisms that underlie this phenomenon remain poorly understood. Previously, we showed that regenerating stem cells in the axolotl spinal cord revert to a molecular state resembling embryonic neuroepithelial cells and functionally acquire rapid proliferative divisions ( 〈 xref ref-type="bibr" rid="bib21" 〉 Rodrigo Albors et al., 2015 〈 /xref 〉 ). Here, we refine the analysis of cell proliferation in space and time and identify a high-proliferation zone in the regenerating spinal cord that shifts posteriorly over time. By tracking sparsely-labeled cells, we also quantify cell influx into the regenerate. Taking a mathematical modeling approach, we integrate these quantitative datasets of cell proliferation, neural stem cell activation and cell influx, to predict regenerative tissue outgrowth. Our model shows that while cell influx and neural stem cell activation play a minor role, the acceleration of the cell cycle is the major driver of regenerative spinal cord outgrowth in axolotls.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.20357.001
DOI:
10.7554/eLife.20357.002
DOI:
10.7554/eLife.20357.003
DOI:
10.7554/eLife.20357.004
DOI:
10.7554/eLife.20357.005
DOI:
10.7554/eLife.20357.006
DOI:
10.7554/eLife.20357.007
DOI:
10.7554/eLife.20357.008
DOI:
10.7554/eLife.20357.009
DOI:
10.7554/eLife.20357.010
DOI:
10.7554/eLife.20357.011
DOI:
10.7554/eLife.20357.012
DOI:
10.7554/eLife.20357.013
DOI:
10.7554/eLife.20357.021
DOI:
10.7554/eLife.20357.022
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2016
detail.hit.zdb_id:
2687154-3
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