In:
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, American Physiological Society, Vol. 317, No. 1 ( 2019-07-01), p. R190-R202
Abstract:
Proinflammatory cytokines like interleukin-1β (IL-1β) affect the control of breathing. Our aim is to determine the effect of the anti-inflammatory cytokine IL-10 οn the control of breathing. IL-10 knockout mice (IL-10 −/− , n = 10) and wild-type mice (IL-10 +/+ , n = 10) were exposed to the following test gases: hyperoxic hypercapnia 7% CO 2 -93% O 2 , normoxic hypercapnia 7% CO 2 -21% O 2 , hypoxic hypercapnia 7% CO 2 -10% O 2 , and hypoxic normocapnia 3% CO 2 -10% O 2 . The ventilatory function was assessed using whole body plethysmography. Recombinant mouse IL-10 (rIL-10; 10 μg/kg) was administered intraperitoneally to wild-type mice ( n = 10) 30 min before the onset of gas challenge. IL-10 was administered in neonatal medullary slices (10–30 ng/ml, n = 8). We found that IL-10 −/− mice exhibited consistently increased frequency and reduced tidal volume compared with IL-10 +/+ mice during room air breathing and in all test gases (by 23.62 to 33.2%, P 〈 0.05 and −36.23 to −41.69%, P 〈 0.05, respectively). In all inspired gases, the minute ventilation of IL-10 −/− mice was lower than IL-10 +/+ (by −15.67 to −22.74%, P 〈 0.05). The rapid shallow breathing index was higher in IL-10 −/− mice compared with IL-10 +/+ mice in all inspired gases (by 50.25 to 57.5%, P 〈 0.05). The intraperitoneal injection of rIL-10 caused reduction of the respiratory rate and augmentation of the tidal volume in room air and also in all inspired gases (by −12.22 to −29.53 and 32.18 to 45.11%, P 〈 0.05, respectively). IL-10 administration in neonatal rat ( n = 8) in vitro rhythmically active medullary slice preparations did not affect either rhythmicity or peak amplitude of hypoglossal nerve discharge. In conclusion, IL-10 may induce a slower and deeper pattern of breathing.
Type of Medium:
Online Resource
ISSN:
0363-6119
,
1522-1490
DOI:
10.1152/ajpregu.00065.2019
Language:
English
Publisher:
American Physiological Society
Publication Date:
2019
detail.hit.zdb_id:
1477297-8
SSG:
12
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