In:
Functional Plant Biology, CSIRO Publishing, Vol. 25, No. 1 ( 1998), p. 39-
Abstract:
Three selectable marker genes were compared for their efficacy in the
production of transgenic wheat plants following microprojectile bombardment of cultured immature embryos. While transformed plants were recovered using the
bar (phosphinothricin acetyltransferase) gene in combination with bialaphos, and the aphA (neomycin
phosphotransferase) gene in combination with geneticin or paromomycin, no transgenic material was obtained with the hpt
(hygromycin phosphotransferase) gene and hygromycin B. Southern analysis revealed single copy as well as multiple copy insertions of the
bar and aphA transgenes. Inheritance of these selectable marker genes was demonstrated in the
T1 generation progenies.
Type of Medium:
Online Resource
ISSN:
1445-4408
Language:
English
Publisher:
CSIRO Publishing
Publication Date:
1998
detail.hit.zdb_id:
2071582-1
SSG:
12
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