In:
Acta Physiologica, Wiley, Vol. 231, No. 3 ( 2021-03)
Abstract:
Metabolic sources switch from carbohydrates in utero , to fatty acids after birth and then a mix once adults. O ‐GlcNAcylation ( O ‐GlcNAc) is a post‐translational modification considered as a nutrient sensor. The purpose of this work was to assess changes in protein O ‐GlcNAc levels, regulatory enzymes and metabolites during the first periods of life and decipher the impact of O ‐GlcNAcylation on cardiac proteins. Methods Heart, brain and liver were harvested from rats before and after birth (D‐1 and D0), in suckling animals (D12), after weaning with a standard (D28) or a low‐carbohydrate diet (D28F), and adults (D84). O ‐GlcNAc levels and regulatory enzymes were evaluated by western blots. Mass spectrometry (MS) approaches were performed to quantify levels of metabolites regulating O ‐GlcNAc and identify putative cardiac O ‐GlcNAcylated proteins. Results Protein O ‐GlcNAc levels decrease drastically and progressively from D‐1 to D84 (13‐fold, P 〈 .05) in the heart, whereas the changes were opposite in liver and brain. O ‐GlcNAc levels were unaffected by weaning diet in any tissues. Changes in expression of enzymes and levels of metabolites regulating O ‐GlcNAc were tissue‐dependent. MS analyses identified changes in putative cardiac O ‐GlcNAcylated proteins, namely those involved in the stress response and energy metabolism, such as ACAT1, which is only O ‐GlcNAcylated at D0. Conclusion Our results demonstrate that protein O ‐GlcNAc levels are not linked to dietary intake and regulated in a time and tissue‐specific manner during postnatal development. We have identified by untargeted MS putative proteins with a particular O ‐GlcNAc signature across the development process suggesting specific role of these proteins.
Type of Medium:
Online Resource
ISSN:
1748-1708
,
1748-1716
DOI:
10.1111/apha.2021.231.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2021
detail.hit.zdb_id:
2617148-X
detail.hit.zdb_id:
2219379-0
SSG:
12
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