In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 16, No. 12 ( 2020-12-11), p. e1009186-
Abstract:
Cells are exposed to frequent mechanical and/or chemical stressors that can compromise the integrity of the plasma membrane and underlying cortical cytoskeleton. The molecular mechanisms driving the immediate repair response launched to restore the cell cortex and circumvent cell death are largely unknown. Using microarrays and drug-inhibition studies to assess gene expression, we find that initiation of cell wound repair in the Drosophila model is dependent on translation, whereas transcription is required for subsequent steps. We identified 253 genes whose expression is up-regulated (80) or down-regulated (173) in response to laser wounding. A subset of these genes were validated using RNAi knockdowns and exhibit aberrant actomyosin ring assembly and/or actin remodeling defects. Strikingly, we find that the canonical insulin signaling pathway controls actin dynamics through the actin regulators Girdin and Chickadee (profilin), and its disruption leads to abnormal wound repair. Our results provide new insight for understanding how cell wound repair proceeds in healthy individuals and those with diseases involving wound healing deficiencies.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1009186
DOI:
10.1371/journal.pgen.1009186.g001
DOI:
10.1371/journal.pgen.1009186.g002
DOI:
10.1371/journal.pgen.1009186.g003
DOI:
10.1371/journal.pgen.1009186.g004
DOI:
10.1371/journal.pgen.1009186.g005
DOI:
10.1371/journal.pgen.1009186.g006
DOI:
10.1371/journal.pgen.1009186.g007
DOI:
10.1371/journal.pgen.1009186.g008
DOI:
10.1371/journal.pgen.1009186.g009
DOI:
10.1371/journal.pgen.1009186.g010
DOI:
10.1371/journal.pgen.1009186.t001
DOI:
10.1371/journal.pgen.1009186.s001
DOI:
10.1371/journal.pgen.1009186.s002
DOI:
10.1371/journal.pgen.1009186.s003
DOI:
10.1371/journal.pgen.1009186.s004
DOI:
10.1371/journal.pgen.1009186.s005
DOI:
10.1371/journal.pgen.1009186.s006
DOI:
10.1371/journal.pgen.1009186.s007
DOI:
10.1371/journal.pgen.1009186.s008
DOI:
10.1371/journal.pgen.1009186.s009
DOI:
10.1371/journal.pgen.1009186.s010
DOI:
10.1371/journal.pgen.1009186.s011
DOI:
10.1371/journal.pgen.1009186.s012
DOI:
10.1371/journal.pgen.1009186.r001
DOI:
10.1371/journal.pgen.1009186.r002
DOI:
10.1371/journal.pgen.1009186.r003
DOI:
10.1371/journal.pgen.1009186.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2186725-2
Permalink