In:
Arhiv za bioloske nauke, National Library of Serbia, Vol. 64, No. 4 ( 2012), p. 1319-1326
Abstract:
Although the genotype and subgenotype distribution of HBV isolates from
Serbia has previously been reported, data about whole genome sequences from
this area are scarce. This study included plasma samples from 5 chronically
infected patients. Full genome amplification of the HBV isolates was
performed by nested-PCR using 7 primer pairs, and the whole genome nucleotide
sequences were obtained by direct sequencing. Two complete genome sequences
belonged to D2 subgenotype (ayw3 HBsAg subtype), one to D1 (ayw2) and two to
A2 (adw2). All 5 Serbian isolates clustered with sequences from the expected
geographic regions and had nucleotide and coded protein length in accordance
to their assigned genotypes, except for one HBeAg-negative isolate displaying
G1896A mutation leading to a premature stop codon in the Pre-C region. The
first complete genome sequences of HBV D1, D2 and A2 subgenotypes from
Serbian patients showed characteristics similar to the nucleotide sequences
of HBV isolates from other European and Middle East countries.
Type of Medium:
Online Resource
ISSN:
0354-4664
,
1821-4339
Language:
English
Publisher:
National Library of Serbia
Publication Date:
2012
detail.hit.zdb_id:
2380842-1
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